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Isolation of a clone partially encoding hill kangaroo X-linked hypoxanthine phosphoribosyltransferase: Sex differences in methylation in the body of the gene

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Somatic Cell and Molecular Genetics

Abstract

An X-linked clone encoding exons 4–9 of the hypoxanthine phosphoribosyltransferase (HPRT) gene was isolated from a kangaroo (Macropus robustus: Marsupialia) λEMBL4 genomic library. Sequence similarity between the kangaroo and eutherian HPRT coding sequences was high; however, intron sizes varied significantly between the kangaroo and other eutherian species. HpaII and HhaI sites in the body of the gene were generally hypermethylated in vivo on the active, relative to the inactive X, with sites within intron 3 showing essentially complete correspondence of activity with methylation and inactivity with unmethylation. At approximately 5 kb downstream from the gene, a switch to unmethylation of active X-linked sites occurred. This switch occurred within a cluster of HpaII and HhaI sites that may represent a CG island associated with a subsequent gene.

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Authors and Affiliations

  1. School of Biological and Biomedical Sciences, University of Technology Sydney, 2065, New South Wales, Australia

    Anita A. Piper, A. M. Bennett, L. Noyce & M. K. Swanton

  2. School of Biological Sciences, Macquarie University, 2109, New South Wales, Australia

    D. W. Cooper

Authors
  1. Anita A. Piper
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  2. A. M. Bennett
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  3. L. Noyce
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  4. M. K. Swanton
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  5. D. W. Cooper
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Additional information

The sequence data in this paper have been submitted to the Genbank Data library under the accession numbers M77 683; M77 684; M77 685; M77 686.

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Piper, A.A., Bennett, A.M., Noyce, L. et al. Isolation of a clone partially encoding hill kangaroo X-linked hypoxanthine phosphoribosyltransferase: Sex differences in methylation in the body of the gene. Somat Cell Mol Genet 19, 141–159 (1993). https://doi.org/10.1007/BF01233530

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  • DOI: https://doi.org/10.1007/BF01233530

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