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Multiple regulatory elements ensure accurate transcription of a human ribosomal protein gene

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Somatic Cell and Molecular Genetics

Abstract

Previously we have shown that expression of a cloned human ribosomal protein gene,RPS14, depends upon regulatory sites located within the gene's proximal upstream DNA plus its first intron. In order to identifycis-active sequence motifs within theRPS14 promoter-enhancer complex, we transiently expressed a set of informative deletion clones in cultured Chinese hamster ovary cells. These experiments revealed three DNA sequence motifs that surround the S14 mRNA initiation site and are necessary for accurate transcription. Electrophoretic mobility shift, DNase I footprint, and methylation interference assays resolved two nuclear proteins, NFα-1 and NFβ-1, which bind specifically to these regulatory motifs. NF-α1 recognizes a pair of 6-bp target motifs (5′-TTCCGG-3′) that flank the 5′ end ofRPS14 exon I; and NF-β1 binds to a 10-bp target sequence (5′-CCGTGGGAAC-3′) within the gene's first intron. Site-directed deletion mutations within the NF-α1 and -β1 binding sites markedly inhibit S14 mRNA transcription.

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  1. Douglas D. Rhoads

    Present address: Department of Biological Sciences, University of Arkansas, 72701, Fayetteville, Arkansas

Authors and Affiliations

  1. Division of Biology, Kansas State University, 66506, Manhattan, Kansas

    Paul F. Overman, Douglas D. Rhoads, Elena S. Tasheva, Marla M. Pyle & Donald J. Roufa

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Overman, P.F., Rhoads, D.D., Tasheva, E.S. et al. Multiple regulatory elements ensure accurate transcription of a human ribosomal protein gene. Somat Cell Mol Genet 19, 347–362 (1993). https://doi.org/10.1007/BF01232747

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  • DOI: https://doi.org/10.1007/BF01232747

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