Summary
Insulin treated patients frequently develop insulin-binding antibody. Antisera from some patients, treated with bovine or bovine/porcine insulin mixtures, react differently with bovine and porcine insulin while for others there is no difference: when there is a difference there is greater avidity for bovine than porcine insulin. We studied antisera from 16 patients who had previously been treated with bovine insulin and then were changed to porcine insulin. Dissociation rate constants and association rates were measured with bovine and porcine insulin. Significant differences were found in association rates, which correlated well with binding at equilibrium (r=0.91), but not in dissociation rates. For 11 subjects who stayed on highly purified porcine insulin, long term reduction in insulin requirement correlated with the magnitude of the difference in antibody reaction to bovine and porcine insulin (r=0.74).
Article PDF
Similar content being viewed by others
References
Lowell, F.C.: Immunological studies in insulin resistance. J. Clin. Invest.23, 225–240 (1944)
Berson, S.A., Yalow, R.S.: Species-specificity of human antibeef, pork insulin serum. J. Clin. Invest.38, 2017–2025 (1959)
Boshell, B.R., Barrett, J.C., Wilensky, S., Patton, T.B.: Insulin resistance. Diabetes13, 144–152 (1964)
Devlin, J.G., Brien, T.G.: Relationship between differential antibody binding capacity and clinical requirements of beef and pork insulin. Metabolism14, 1034–1036 (1965)
de Mowbray, R.R.: Risk of changing from ox to pig insulin. Lancet1966 II, 1027–1028
Asplin, C.M., Hartog, M.: Hazards of mono-component insulin. Br. Med. J.1976 I, 1146
Logie, A.W., Stowers, J.M.: Hazards of monocomponent insulin. Br. Med. J.1976 I, 879–880
Andreani, D., Lavicoli, M., Tamburrano, G., Menzinger, G.: Comparative trials with monocomponent (MC) and mono-species (MS) pork insulins in the treatment of diabetes mellitus. Horm. Metab. Res.6, 447–454 (1974)
Mustaffa, B.E., Daggett, P.R., Nabarro, J.D.N.: Insulin binding capacity in patients changed from conventional to highly purified insulins. Diabetologia13, 311–315 (1977)
Asplin, C.M., Hartog, M., Goldie, D.J.: Change of insulin dosage, circulating free and bound insulin and insulin antibodies on transferring diabetics from conventional to highly purified porcine insulin. Diabetologia14, 99–105 (1978)
Kurtz, A.B., Mustaffa, B.E., Daggett, P.R., Nabarro, J.D.N.: Effect of insulin antibodies on free and total plasmainsulin. Lancet1977 II, 56–58
Gavin, J.R., Roth, J., Jen, P., Freychet, P.: Insulin receptors in human circulating cells and fibroblasts. Proc. Natl. Acad. Sci. USA69, 747–751 (1972)
Andersen, O.O., Brunfeldt, K., Abilgard, F.: A method for quantitative determination of insulin antibodies in human plasma. Acta Endocrinol. (Kbh.)69, 195–208 (1972)
Berson, S.A., Yalow, R.S.: Quantitative aspects of the reaction between insulin and insulin-binding antibody. J. Clin. Invest.38, 1996–2016 (1959)
Andersen, O.O.: Insulin antibody formation. Acta Endocrinol. (Kbh.)72, 33–45 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kurtz, A.B., Matthews, J.A. & Nabarro, J.D.N. Insulin-binding antibody: Reaction differences with bovine and porcine insulins. Diabetologia 15, 19–22 (1978). https://doi.org/10.1007/BF01219322
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01219322