Summary
The insulin response to glucose stimulation has been investigatedin vivo andin vitro in suckling rats from small or large litters. In two day old animals from small litters there was a marked increase in serum insulin levels after an intraperitoneal glucose injection, while the insulin response to glucose in animals from large litters was low and sluggish. The weak insulin response to glucose in the two day old rats from large litters was further substantiated in studiesin vitro in which the insulin release of isolated pancreatic glands was measured after incubation in a low or a high glucose concentration. On the third and fourth postnatal days there was an increased insulin secretion in response to the glucose challenge bothin vivo andin vitro in the two groups of animals. The results indicate that postnatal feeding modulates the development of the glucose-mediated insulin release.
Résumé
La réponse de l'insuline à une stimulation par le glucose a été étudiéein vivo etin vitro chez des rats nouveau-nés de petites ou de grandes portées. Chez les animaux des petites portées, âgés de deux jours, il y avait une nette augmentation des taux de l'insuline du sérum après une injection de glucose intrapéritonéale, tandis que la réponse de l'insuline au glucose était faible et lente chez les animaux des grandes portées, âgés de deux jours. La faible réponse de l'insuline au glucose chez les animaux des grandes portées, âgés de deux jours, a été confirmée par l'étudein vitro dans laquelle a été mesurée la sécrétion d'insuline par les glandes pancréatiques isolées après incubation dans une concentration faible ou forte de glucose. Le 3e ou le 4e jour après la naissance, il y a une augmentation de la sécrétion d'insuline en réponse à une charge de glucosein vivo etin vitro dans les deux groupes d'animaux. — Les résultats indiquent que la nutrition post-natale influence le développement de la sécrétion d'insuline provoquée par le glucose. La possibilité d'une influence des facteurs extra-pancréatiques sur le procédé de maturation est discutée.
Zusammenfassung
Die Insulinsekretion nach Glucosestimulation wurdein vivo undin vitro an kleinen und großen Würfen säugender Ratten untersucht. Bei 2 Tage alten Tieren aus kleinen Würfen war ein starker Anstieg des Seruminsulinspiegels nach intraperitonealer Glucoseinjektion festzustellen, während die Insulinantwort auf Glucose bei Tieren eines großen Wurfs langsam und gering war. Die schwache Insulinsekretion nach Glucose bei 2 Tage alten Ratten eines großen Wurfs wurdein vitro weiter untersucht und die Insulinsekretion in geringer und hoher Glucosekonzentration gemessen. Am dritten und vierten postnatalen Tag wurde eine erhöhte Insulinsekretionin vivo undin vitro bei beiden Tiergruppen beobachtet. Die Ergebnisse deuten darauf hin, daß die Entwicklung der glucoseinduzierten Insulinsekretion von der postnatalen Fütterung beeinflußt ist.
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Asplund, K. Effects of postnatal feeding on the functional maturation of pancreatic islet B-cells of neonatal rats. Diabetologia 8, 153–159 (1972). https://doi.org/10.1007/BF01212254
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DOI: https://doi.org/10.1007/BF01212254