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Electron microscopic localization of [125I]α-bungarotoxin binding sites in the outer plexiform layer of the goldfish retina

  • Published:
Journal of Neurocytology

Summary

Light and electron microscope autoradiography were performed on goldfish (Carassius auratus) retinas incubated in [125I] labelled α-bungarotoxin. The toxin was bound preferentially to membrane receptors in the inner and outer plexiform layers. Binding was suppressed by 10−5 M nicotine or 10−5 M native α-bungarotoxin. Electron microscopic analysis of the outer plexiform layer (OPL) strongly suggested that α-bungarotoxin binding sites were located on small bipolar cell dendritic processes that invaginated rod and cone synaptic terminals, and on large bipolar cell dendritic processes more proximally situated in the OPL. Large horizontal cell processes in the OPL and horizontal cell processes that invaginated rod and cone synaptic terminals did not appear to be labelled.

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  1. I. R. Schwartz

    Present address: Department of Surgery/32-34 Rehabilitation Center, UCLA School of Medicine, 90024, Los Angeles, California, USA

Authors and Affiliations

  1. Department of Anatomy and the Jules Stein Eye Institute, UCLA School for Medicine, 90024, Los Angeles, California, USA

    I. R. Schwartz & D. Bok

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  1. I. R. Schwartz
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Schwartz, I.R., Bok, D. Electron microscopic localization of [125I]α-bungarotoxin binding sites in the outer plexiform layer of the goldfish retina. J Neurocytol 8, 53–66 (1979). https://doi.org/10.1007/BF01206458

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  • DOI: https://doi.org/10.1007/BF01206458

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