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Review

Transplantation of Fetal Germ Cells

  • Clinical Assisted Reproduction
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Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Oogonial stem cells are short-lived and endow the ovary with its lifetime store of follicles during fetal life. No compensatory mechanisms exist to replace germ cells that are lost for whatever reason after birth. Fetal germ cells and the abundant primordial follicles of immature animals can be successfully stored at low temperatures and transplanted to hosts to generate normal ovulatory cycles. Sterilized hosts are restored to fertility. Such results suggest that the abundant reserves of germ cells in the ovaries of human abortuses offer opportunities for treating patients whose sterility is due to afollicular ovaries uncomplicated by autoimmune disease. The prospects for this treatment depend largely on the vigilance of the recipient's immune system and public attitudes to a radical treatment, though one that promises to overcome sterility and hypoestrogenism in women with either premature menopause or gonadal dysgenesis.

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References

  1. Baker TG: A quantitative and cytological study of germ cells in human ovaries. Proc Roy Soc Lond B 1963;158:414–433

    Google Scholar 

  2. Block E: Quantitative morphological investigations of the follicular system in women. Acta Anat 1952;14:108–123

    PubMed  Google Scholar 

  3. Block E: A quantitative morphological investigation of the follicular system in newborn infants. Acta Anat 1953;17:201–206

    PubMed  Google Scholar 

  4. Richardson SJ, Senikas V, Nelson JF: Follicular depletion during the menopausal transition: Evidence for accelerated loss and ultimate exhaustion. J Clin Endocrinol Metab 1987;65:1231–1237

    PubMed  Google Scholar 

  5. Gosden RG: Biology of Menopause: The Causes and Consequences of Ovarian Ageing. London, Academic Press, 1985, pp 37–63

    Google Scholar 

  6. Coulam CB, Adamson SC, Annegers JF: Incidence of premature ovarian failure. Obstet Gynecol 1986;67:604–606

    PubMed  Google Scholar 

  7. Cornet D, Alvarez S, Antoine JM, Tibi Ch, Mandelbaum J, Plachot M, Salat-Baroux J: Pregnancies following ovum donation in gonadal dysgenesis. Hum Reprod 1990;5:291–293

    PubMed  Google Scholar 

  8. Rotszteyn DA, Remohi J, Weckstein LN, Ord T, Moyer DL, Balmaceda JP, Asch RH: Results of tubal embryo transfer in premature ovarian failure. Fertil Steril 1990;54:348–350

    PubMed  Google Scholar 

  9. Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK: Pregnancy afterin vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109–113

    PubMed  Google Scholar 

  10. Krohn PL: Transplantation of the ovary.In The Ovary, Vol II. Physiology, Lord Zuckerman, BJ Weir (eds). New York and London, Academic Press, 1977, pp 101–128

    Google Scholar 

  11. Gosden RG: Transplantation of ovaries and testes.In Fetal Transplants in Medicine, RG Edwards (ed). Cambridge, Cambridge University Press, 1992 (in press)

    Google Scholar 

  12. Winston RML, McClure Browne JC: Pregnancy following autograft transplantation of fallopian tube and ovary in the rabbit. Lancet 1974;2:494–495

    PubMed  Google Scholar 

  13. Cornier E, Sibella P, Chatelet F: Etudes histologiques et devenir fonctionnel des greffes de trompe et d'ovaire chez la rate (isogreffes et allogreffes traittées par cyclosporine A). J Gynecol Obstet Biol Reprod 1985;14:567–573

    Google Scholar 

  14. Felicio LS, Nelson JF, Gosden RG, Finch CE: Restoration of ovulatory cycles by young ovarian grafts in aging mice: Potentiation by long-term ovariectomy decreases with age. Proc Natl Acad Sci USA 1983;80:6076–6080

    PubMed  Google Scholar 

  15. Krohn PL: Transplantation of endocrine organs. With special reference to the ovary. Br Med Bull 1965;21:157–162

    PubMed  Google Scholar 

  16. Krohn PL: Review lectures on senescence. II. Heterochronic transplantation in the study of ageing. Proc Roy Soc B 1962;157:128–147

    Google Scholar 

  17. Nelson JF, Felicio LS: Reproductive aging in the female: An etiological perspective. Rev Biol Res Aging 1985;2:251–314

    Google Scholar 

  18. Aschheim P: Résultats fournis par la greffe héterochrone des ovaires dans l'étude de la régulation hypothalamo-hypophyso-ovarienne de la ratte sénile. Gerontologia 1965;10:65–75

    Google Scholar 

  19. Mobbs CV, Gee DM, Finch CE: Reproductive senescence in female C57BL/6J mice: Ovarian impairments and neuroendocrine impairments that are partially reversible and delayable by ovariectomy. Endocrinology 1984;115:1653–1662

    PubMed  Google Scholar 

  20. Foa C: La greffe des ovaires en relation avec quelques questions de biologie generale. Arch Ital Biol 1900;34:43–73

    Google Scholar 

  21. Byskov AG: Differentiation of mammalian embryonic gonad. Physiol Rev 1986;66:71–117

    PubMed  Google Scholar 

  22. Arrau J, Roblero L, Cury M, Gonzalez, R: Effect of exogenous sex steroids upon the number of germ cells and the growth of foetal ovaries grafted under the kidney capsule of adult ovariectomized hamsters. J Embryol Exp Morphol 1983;78:33–42

    PubMed  Google Scholar 

  23. Burgoyne PS, Ansell JD, Tournay A: Can the indifferent mammalian XX gonad be sex-reversed by interaction with testicular tissue?In Development and Function of the Reproductive Organs, A Eshkol, B Eckstein, N Dekel, H Peters, A Tsafriri (eds). Rome, Ares-Serono Symposium No. 11, 1983, pp 23–39

  24. Gosden RG: Unpublished observations

  25. Taketo-Hosotani T, Merchant-Larios H, Thau RB, Koide SS: Testicular cell differentiation in fetal mouse ovaries following transplantation into adult male mice. J Exp Zool 1985;236:229–237

    PubMed  Google Scholar 

  26. Greenwald GS, Moor RM: Isolation and preliminary characterization of pig primordial follicles. J Reprod Fert 1989;85:561–571

    Google Scholar 

  27. Torrance C, Telfer E, Gosden RG: Quantitative study of the development of isolated mouse pre-antral follicles in collagen gel culture. J Reprod Fert 1989;87:367–374

    Google Scholar 

  28. Telfer E, Torrance C, Gosden RG: Morphological study of cultured preantral ovarian follicles after transplantation under the kidney capsule. J Reprod Fert 1990;89:565–571

    Google Scholar 

  29. Gosden RG: Restitution of fertility in sterilized mice by transferring primordial ovarian follicles. Hum Reprod 1990;5:499–504

    PubMed  Google Scholar 

  30. Parkes AS, Smith AU: Regeneration of rat ovarian tissue grafted after exposure to low temperatures. Proc Roy Soc B 1953;140:455–470

    Google Scholar 

  31. Green SH, Smith AU, Zuckerman S: The numbers of oocytes in ovarian autografts after freezing and thawing. J Endocr 1956;13:330–334

    PubMed  Google Scholar 

  32. Daniel JC Jr, Fox JM, Richardson P: Return to partial function of rabbit ovaries after cryopreservation in liquid nitrogen. Theriogenology 1983;19:405–411

    Google Scholar 

  33. Parrot DMV: The fertility of mice with orthotopic ovarian grafts derived from frozen tissue. J Reprod Fert 1960;1:230–241

    Google Scholar 

  34. Carroll J, Whittingham DG, Wood MJ, Telfer E, Gosden RG: Extra-ovarian production of mature viable mouse oocytes from frozen primary follicles. J Reprod Fert 1990;90:321–327

    Google Scholar 

  35. Carroll J, Gosden RG: Unpublished results

  36. Woodruff MFA: The Transplantation of Tissues and Organs. Springfield IL, CC Thomas, 1960, pp 494–503

    Google Scholar 

  37. Biggers JD:In vitro fertilization and embryo transfer in historical perspective.In In Vitro Fertilization and Embryo Transfer, A Trounson, C Wood (eds). Edinburgh, Churchill Livingstone, 1984, pp 3–15

    Google Scholar 

  38. Hansen JT, Sladek JR Jr: Fetal research. Science 1989;246:775–779

    PubMed  Google Scholar 

  39. Hollands P: Embryonic stem cell grafting: the therapy of the future? Hum Reprod 1991;6:79–84

    PubMed  Google Scholar 

  40. Edwards RG: Fetal Transplants in Medicine. Cambridge, Cambridge University Press, 1992 (in press)

    Google Scholar 

  41. Polkinghorne J: Review of the guidance on the research use of fetuses and fetal material. London, Her Majesty's Stationery Office, Cm 762, 1989

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University Medical School, Teviot Place, EH8 9AG, Edinburgh, Scotland, UK

    Roger G. Gosden

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  1. Roger G. Gosden
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Gosden, R.G. Review. J Assist Reprod Genet 9, 118–123 (1992). https://doi.org/10.1007/BF01203750

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