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Production of insulin-like growth factor binding proteins by human ovarian carcinoma cells

  • Original Papers
  • Experimental Oncology
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Abstract

Cells of the human ovarian carcinoma lines EFO-21, EFO-27, MFO-35 and MFO-36 secrete binding proteins for insulin-like growth factors (IGFBPs) into their culture media. By sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) and ligand blotting, seven groups of IGFBPs with molecular masses of 25, 30 (doublet), 34, 37, 40, 45, and 50 kDa were observed, depending on the cell line under investigation. By Northern blot analyses using cDNAs or oligonucleotides specific for the six types of IGFBP (IGFBP-1 to IGFBP-6), mRNA for all IGFBPs tested except for IGFBP-1 could be detected in the ovarian carcinoma cell extracts. In detail, analysis of EFO-21 protein products by SDS-PAGE yielded IGFBPs of 25, 34, and 50 kDa; extracts of EFO-21 cells contained mRNAs for IGFBP-2, -3, -4, and -6. EFO-27 cells produced IGFBPs of 40 kDa and 45 kDa as determined by SDS-PAGE, and mRNAs for IGFBP-3, -4, and -6 were detected. In the conditioned medium of MFO-35 cells, IGFBPs of 25, 30 (doublet), 34, 37, 40, and 45 kDa were observed by SDS-PAGE, while mRNAs for the five proteins IGFBP-2 to IGFBP-6 were found. MFO-36 cells produced IGFBPs of 34 kDa and 50 kDa as determined by SDS-PAGE, and the cells expressed mRNAs for IGFBP-2, -3, -4, and -6. In relation to published molecular mass data of the known IGFBPs, the size of the secreted proteins could be correlated to the mRNA patterns expressed by the ovarian carcinoma cells. It is concluded that ovarian carcinoma cells frequently express IGFBP-3, -4, and -6 and, to a lesser extent, IGFBP-2; the expression of IGFBP-5 appears as a rather rare event, while IGFBP-1 was not found to be expressed in ovarian carcinoma cells.

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Abbreviations

BSA:

bovine serum albumin

FCS:

fetal bovine serum

PBS:

phosphate-buffered saline

SCC:

citrate-buffered saline

SFM:

serum-free medium

TRIS/NaCl:

TRIS-buffered saline

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Authors and Affiliations

  1. Department of Internal Medicine, Section Hematology/Oncology/Immunology, Philipps-University, Baldingerstrasse, D-35033, Marburg, Germany

    Jürgen Hofmann, Barbara Wegmann & Klaus Havemann

  2. Department of Obstetrics and Gynecology, Philipps-University, Marburg, Germany

    Jürgen Hofmann, Reinhard Hackenberg & Klaus-Dieter Schulz

  3. Department of Internal Medicine I, Albert-Ludwigs-University, Freiburg, Germany

    Regina Kunzmann

Authors
  1. Jürgen Hofmann
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  2. Barbara Wegmann
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  3. Reinhard Hackenberg
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  4. Regina Kunzmann
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  5. Klaus-Dieter Schulz
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  6. Klaus Havemann
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Additional information

Supported by the Deutsche Forschungsgemeinschaft, Bonn, Germany (SFB 215, project Al), and by the Bundesministerium für Forschung und Technologie, Bonn, Germany (BMFT grant 01GA8715/9)

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Hofmann, J., Wegmann, B., Hackenberg, R. et al. Production of insulin-like growth factor binding proteins by human ovarian carcinoma cells. J Cancer Res Clin Oncol 120, 137–142 (1994). https://doi.org/10.1007/BF01202191

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  • DOI: https://doi.org/10.1007/BF01202191

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