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Purification of glucoamylase fromAspergillus terreus

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Abstract

Glucoamylase from a rice bran culture ofAspergillus terreus was purified by chromatography on DEAE-cellulose and concanavatin A-Sepharose. A homogenous monomer resulted after SDS-PAGE electrophoresis. The enzyme was a glycoprotein, molecular weight, 86,000 with 7.5% (w/w) carbohydrate content.

Résumé

La glucoamylase d'Aspergillus terreus cultivee sur son de riz a été purifiée par chromatographie sur DEAE-cellulose et sur A-sepharose à concavaline. On a obtenu un monomère homogène après électrophorèse sur SDS-PAGE. L'enzyme est une glycoproténe d'un poids moléculaire de 86,000 avec un contenu en polysacchardie de 7.5% (p/p).

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References

  • Ali, S., Mahmood, S., Alam, R. &Hossain, Z. 1989 Culture condition for production of glucoamylase from rice bran byAspergillus terreus.MIRCEN Journal of Applied Microbiology and Biotechnology 5, 121–127.

    Google Scholar 

  • Charuchinda, A., Laopaiboon, R., Chaisrisook, C., Lotong, N. & Yamamoto, K. 1984 Glucoamylase of citric acid producing mutant ofAspergillus niger. InMicrobial Utilization of Renewable Resources, Vol. 4, ed. Taguchi, H. pp. 101–106, Osaka University.

  • Dubois, M., Gillis, K.A., Hamilton, J.K., Rebers, P.A. &Smith, F. 1956 Colorimetric method for the determination of sugar and related substances.Analytical Chemistry 28, 350–356.

    Google Scholar 

  • Fogarty, W.M. 1983Microbial amylases InMicrobial Enzymes and Biotechnology, ed. Fogarty, W.M. pp. 1–92. London: Applied Science.

    Google Scholar 

  • Goldstein, I.J., Hollerman, C.E. &Smith, I.E. 1965 Protein-carbohydrate interaction—II. Inhibition studies in the interaction of Concanavallin A with polysaccharides.Biochemistry 4, 876–887.

    Google Scholar 

  • Kanlayakrit, W., Ishimatsu, K., Nakao, M. &Hayashida, S. 1987 Characteristics of raw starch digesting glucoamylase fromRhizomucor pusillus.Journal of Fermentation Technology 65, 379–385.

    Google Scholar 

  • Laemmili, U.K. 1975 Cleavage of structural protein during the head of bacteriophage T4.Nature (London) 227, 680–685.

    Google Scholar 

  • Manjunath, P., Shenoy, B.C. &Raghuvendra Rao, M.R. 1983 Fungal glucoamylases.Journal of Applied Chemistry 5, 235–260.

    Google Scholar 

  • Muthukumuran, N. &Dhar, S.C. 1983 Purification and properties of glucoamylase fraction from the culture filtrate ofRhizopus nodusus.Italian Journal of Biochemistry 32, 239–253.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Dhaka, 1000, Dhaka, Bangladesh

    S. Ali, Z. Hossain, S. Mahmood & R. Alam

Authors
  1. S. Ali
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  2. Z. Hossain
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  3. S. Mahmood
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  4. R. Alam
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Ali, S., Hossain, Z., Mahmood, S. et al. Purification of glucoamylase fromAspergillus terreus . World J Microbiol Biotechnol 6, 431–433 (1990). https://doi.org/10.1007/BF01202129

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  • DOI: https://doi.org/10.1007/BF01202129

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