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The effect of a mesogenic and a lentogenic Newcastle disease virus strain on Burkitt lymphoma Daudi cells

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Abstract

The destructive effect of Newcastle disease virus (NDV) strains on Burkitt lymphoma Daudi cells was investigated. Interaction of an active and UV-inactivated mesogenic strain (Roakin), as well as an active attenuated lentogenic strain (B1), grown in the allantoic sac of embryonated eggs, at high multiplicity, caused inhibition in cellular DNA synthesis and arrest in cell multiplication, eventually killing of the cells. The lentogenic strain cultivated in chicken fibroblasts exhibited only a moderate activity. The mechanism of the cytolytic effect is presumably linked to the increase in cell membrane permeability indicated by the elevation in51Cr release. Thus it appears that the massive adsorption and/or penetration of viral particles, active or UV-inactivated (or possibly a toxic component that resides in the virion), damages the plasma membrane and may be responsible for the killing of the cells.

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Abbreviations

CEF :

chicken embryo fibroblasts

NDV :

Newcastle disease virus

B1 :

attenuated lentogenic NDV strain B1 V-188

RO :

mesogenic (medium virulent) NDV strain Roakin/46 V-109 NJ

CEF-B1 :

B1 cultivated in CEF

RO-IRR :

UV-irradiated RO

m.o.i. :

multiplicity of infection

EID 50 :

median egg-infective dose

PBS :

phosphate-buffered saline

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Authors and Affiliations

  1. Department of Virology, Hebrew University, Hadassah Medical School, P.O.B. 12272, 91120, Jerusalem, Israel

    Y. Tzadok-David, M. Metzkin-Eizenberg & Z. Zakay-Rones

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  1. Y. Tzadok-David
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  2. M. Metzkin-Eizenberg
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  3. Z. Zakay-Rones
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Tzadok-David, Y., Metzkin-Eizenberg, M. & Zakay-Rones, Z. The effect of a mesogenic and a lentogenic Newcastle disease virus strain on Burkitt lymphoma Daudi cells. J Cancer Res Clin Oncol 121, 169–174 (1995). https://doi.org/10.1007/BF01198099

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  • DOI: https://doi.org/10.1007/BF01198099

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