Abstract
Microbial lipids produced byRhodotorula glutinis grown in continuous culture with molasses under nitrogen-limiting conditions were evaluated and the effects of growth rate on fatty acid composition were studied. As the growth rate decreased, cell biomass, lipid content and lipid yield gradually increased. The maximum lipid content recorded was 39% (w/w) of dry cell biomass at a dilution rate of 0.04 h−1. The growth rate also affected fatty acid composition: oleic acid decreased with decreasing growth rate while stearic acid increased.
References
Almazan, O., Klibansky, M. & Otero, M.A. 1981 Microbial fat synthesis byRhodolorula glutinis from blackstrap molasses in continuous culture.Biotechnolgoy Letters3, 663–666.
Choi, S.Y., Dewey, D.Y., Ryn & Rhee, J.S. 1982 Production of microbial lipids: Effects of growth rate and oxygen on lipid synthesis and fatty acid composition ofRhodotorula gracilis.Biotechnology and Bioengineering24, 1165–1172.
De Walley, H.C.S. 1949 Testing of molasses and sugar syrup. International Commission for Sugar Analysis 10th Session, Subject 6, pp. 12–26.
Gonzalez, N. & Kopecky, J. 1972 Estudio de la calidad de las levaduras cubanas. Havana: Biotechnological Department, Cuban Research Institute of Sugar Cane By-Products.
Misra, S., Grosh, A. & Dutta, J. 1984 Production and composition of microbial fat fromRhodotorula glutinis.Journal of the Science of Food and Agriculture35, 59–65.
Moreton, R.S. 1988 Physiology of lipid-accumulating yeasts. InSingle Cell Oil, ed Moreton, R.S., pp. 1–32, Harlow, Essex: Longman.
Ratledge, C. 1979 Resource conservation by novel biological processes. I. Grow fats from wastes.Chemical Society Reviews8, 283–296.
Yoon, S.H. & Rhee, J.S. 1983 Lipids from yeast fermentation: Effects of cultural condition on lipid production and its characteristics ofRhodotorula glutinis.Journal of Americal Oil Chemists' Society60, 1281–1286.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alvarez, R.M., Rodríguez, B., Romano, J.M. et al. Lipid accumulation inRhodotorula glutinis on sugar cane molasses in single-stage continuous culture. World J Microbiol Biotechnol 8, 214–215 (1992). https://doi.org/10.1007/BF01195853
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01195853