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Aberrations of theAPC gene in primary breast carcinoma

  • Original Papers
  • Clinical Oncology
  • Published:
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Abstract

Aberrations of theAPC gene, which plays an important role in the genesis of familial adenomatous polyposis and colorectal carcinoma, were investigated in 31 surgical specimens of primary breast carcinoma. These studies utilized the polymerase chain reaction followed by restriction-fragment-length polymorphism and single-strand-conformation polymorphism analyses combined with tumor cell enrichment by cell sorting. Loss of heterozygosity at theAPC locus was detected in 8 (38%) of 21 informative cases, but only 2 (6%) of 31 tumors carried a mutatedAPC gene. Direct DNA sequencing analysis confirmed mutations at codon 1081 (AGC to ATC) resulting in an amino acid substitution of serine for isoleucine, and at codon 1096 (CAG to CAT) resulting in a substitution of glutamine for histidine. There were no significant correlations between the loss of heterozygosity or mutation at theAPC locus and any clinicopathological characteristics. Our present observations suggest that the mutations of theAPC gene may play an important role in the genesis of certain breast carcinomas, and that another tumor-suppressor gene, which is the true target of frequent loss of heterozygosity, may exist near theAPC gene.

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Abbreviations

LOH:

loss of heterozygosity

PCR:

polymerase chain reaction

RFLP:

restriction-fragment-length polymorphism

SSCP:

single-strand-conformation polymorphism

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Authors and Affiliations

  1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, 020, Morioka, Japan

    Masahiro Kashiwaba & Gen Tamura

  2. Department of Surgery, Iwate Medical University School of Medicine, Uchimaru 19-1, 020, Morioka, Japan

    Motoo Ishida

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  1. Masahiro Kashiwaba
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  2. Gen Tamura
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  3. Motoo Ishida
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Kashiwaba, M., Tamura, G. & Ishida, M. Aberrations of theAPC gene in primary breast carcinoma. J Cancer Res Clin Oncol 120, 727–731 (1994). https://doi.org/10.1007/BF01194271

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  • DOI: https://doi.org/10.1007/BF01194271

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