Abstract
DNA fiber autoradiography was used to measure the rate of replication fork progression along replication units in human diploid cells. The rate in different replication units differs very significantly and lies within the range 0.1 to 1.2 μm/min. However, no significant changes were found in the rate of fork movement along single replication units operating during long intervals of S phase. Moreover, the fork progression rate is constant in many replication units of human cells.
Similar content being viewed by others
References
Edenberg, H.J., Huberman, J.A.: Eukaryotic chromosome replication. Ann. Rev. Genet.9, 245–284 (1975)
Housman, D., Huberman, J.A.: Changes in the rate of DNA replication fork movement during S phase in mammalian cells. J. molec. Biol.94, 173–181 (1975)
Lark, K.G., Consigli, R., Toliver, A.: DNA replication in Chinese hamster cells: Evidence for a single replication fork per replicon. J. molec. Biol.58, 873–875 (1971)
Liapunova, N.A., Khaitova, N.M.: The enhancement of replication rate of DNA molecules during S period in human diploid cells. XIV International Congress of Genetics, Abstracts, Part1, 369, Moscow, 1978
Painter, R.B., Schaeffer, A.W.: Variation in the rate of DNA chain growth through the S phase in Hela cells. J. Molec. Biol.58, 289–295 (1971)
Van't Hof, J.: Replicon size and rate of fork movement in early S of higher plant cells (Pisum sativum). Exp. Cell Res.103, 395–403 (1976)
Yurov, Yu.B.: Replication of DNA in primary culture of human hepatocytes. Dokl. Acad. Nauk (USSR)229, 472–474 (1976)
Yurov, Yu.B.: Replication of chromosomal DNA in Chinese hamster cells cultured at various temperatures. Tsitologia (USSR)19, 1064–1066 (1977a)
Yurov, Yu.B.: DNA replication in human diploid cells of different origin. Cell Different.6, 95–104 (1977b)
Yurov, Yu.B.: Replication of chromosomal DNA in cultured abnormal human cells. Hum. Genet.43, 47–52 (1978)
Yurov, Yu.B., Liapunova, N.A.: The units of DNA replication in the mammalian cells: Evidence for a large sizes of replication units. Chromosoma (Berl.)60, 253–267 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yurov, Y.B. The rate of fork movement during DNA replication in mammalian cells. Chromosoma 74, 347–353 (1979). https://doi.org/10.1007/BF01190749
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01190749