Summary
Junctions between the cells in the reticular lamina of the organ of Corti were examined in thin sections and after freeze-fracturing to find a structural basis for the large ionic differences between the endolymph and perilymph. The apices of the cells in the reticular lamina are joined by a band of tight junctions spaced at 140 Å intervals. Beneath this apical band the organization of the tight junctions depends on whether they join a supporting cell and a hair cell, or two supporting cells. At hair cell junctions with supporting cells, there is an extensive labyrinth of tight junctions enclosing lengthy, tortuous passages whose walls are composed of either multiple parallel or single junctions. At appositions between two supporting cells, maculae or fasciae occludentes lie immediately beneath the apical bands of closely spaced tight junctions, near the top of the zonulae adherentia which are characteristic of appositions between supporting cells. The complexes of tight junctions, or zonulae occludentes, between extralaminar supporting cells differ from those in the reticular lamina. The extralaminar cells are joined by a band of four to seven branching, anastomotic tight junctions. Thus, these junctions are like zonulae occludentes in other tissues. The novel organization of the tight junctions in the reticular lamina, different from those between the extralaminar supporting cells, suggests a special role for these junctions in the reticular lamina. Two sizes of gap junctions link, and presumably couple, supporting cells in the reticular lamina.
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References
Angelborg, C. andEngström, H. (1972) Supporting elements in the organ of Corti. I. Fibrillar structures in the supporting cells of the organ of Corti of mammals.Acta Oto-laryngolica Supplement 301, 49–60.
Beagley, H. A. (1965) Acoustic trauma in the guinea pig. II. Electron microscopy including the morphology of cell junctions in the organ of Corti.Acta Oto-laryngolica 60, 479–95.
Békésey, G. Von (1960) Wave motion in the cochlea. InExperiments in Hearing, pp 485–534. New York: McGraw-Hill.
Bennett, M. V. L., Nakajima, Y. andPappas, G. D. (1967) Physiology and ultrastructure of electronic junctions. I. Supramedullary neurons.Journal of Neurophysiology 30, 161–79.
Bosher, S. K. andWarren, R. L. (1968) Observations on the electrochemistry of the cochlear endolymph of the rat: A quantitative study of its electrical potential and ionic composition as determined by means of flame spectrophotometry.Proceedings of the Royal Society of London, Series B 171, 227–47.
Brightman, M. W. andReese, T. S. (1969) Junctions between intimately apposed cell membranes in the vertebrate brain.Journal of Cell Biology 40, 648–77.
Brightman, M. W., Hori, M., Rapaport, S. I., Reese, T. S. andWestergaard, E. (1973) Osmotic opening of tight junctions in cerebral endothelium.Journal of Comparative Neurology 152, 317–26.
Brightman, M. W., Prescott, L. andReese, T. S. (1975) Intercellular junctions of special ependyma. InBrain Endocrine Interaction. II. The Ventricular System in Neuroendocrine Mechanisms (edited byKnigge, K. M., Scott, D. E. andKobayashi, H.) pp 146–65. Basel: S. Karger.
Brightman, M. W. andRobinson, J. S. (1976) Some attempts to open the blood-brain barrier to protein. InHead Injuries — Second Chicago Symposium on Neural Trauma (edited byMcLaurin, R. L.) New York: Grune and Stratton (In press).
Brightman, M. W., Shivers, R. R. andPrescott, L. (1975) Morphology of the walls around fluid compartments in nervous tissue. InFluid Environment of the Brain, pp 3–29. New York: Academic Press.
Chalcroft, J. P. andBullivant, S. (1970) An interpretation of liver cell membrane and junction structure based on observation of freeze-fracture replicas of both sides of the fracture.Journal of Cell Biology 47, 49–60.
Claude, P. andGoodenough, D. A. (1973) Fracture faces of zonulae occludentes from ‘tight’ and ‘leaky’ epithelia.Journal of Cell Biology 58, 390–400.
De Lorenzo, A. J. D., Shiroky, D. V. andCohn, E. I. (1972) Distribution of exogenous horseradish peroxidase in perilymphatic and endolymphatic spaces of the guinea pig cochlea. InVascular Disorders and Hearing Defects (edited byDe Lorenzo, A. J. D.), pp 187–203. Baltimore: University Park Press.
Davis, H. (1959) Excitation of auditory receptors. InHandbook of Physiology — Neurophysiology I, pp. 565–84. Washington: American Physiological Society.
Dermietzel, R. (1974) Junctions in the central nervous system of the cat. 1. Membrane fusion in central myelin.Cell and Tissue Research 148, 565–76.
Di Bona, D. R. (1972) Passive intercellular pathway in amphibian epithelia.Nature New Biology 238, 179–81.
Di Bona, D. R. andCivan, M. M. (1973) Pathways for movement of ions and water across toad urinary bladder.Journal of Membrane Biology 12, 101–28.
Dunn, R. A. andMorest, D. K. (1975) Receptor synapses without synaptic ribbons in the cochlea of the cat.Proceedings of the National Academy of Sciences USA 72, 3599–3603.
Duvall, A. J. andSutherland, C. R. (1972) Cochlear transport of horseradish peroxidase.Annals of Otology, Rhinology and Laryngology 81, 705–13.
Farquhar, M. G. andPalade, G. E. (1963) Junctional complexes in various epithelia.Journal of Cell Biology 17, 375–412.
Franke, K. D., Reale, E., Iurato, S., Luciano, L., Wermbter, G. andPannese, E. (1975) Verbindungskomplexe an Zellen der Reissner-Membran in gefriergebrochenen Präparaten,Acta Oto-Laryngologica 80, 38–42.
Friend, D. S. andGilula, N. B. (1972) Variations in tight and gap junctions in mammalian tissues.Journal of Cell Biology 53, 758–76.
Gulley, R. L. andReese, T. S. (1975) Intercellular junctions in the reticular lamina of the organ of Corti.Journal of Cell Biology 67, 148a.
Hull, B. E. andStaehelin, L. A. (1976) Functional significance of variations in the geometrical organization of tight junction networks.Journal of Cell Biology 68, 688–704.
Hudspeth, A. J. andYee, A. G. (1973) The intercellular junctional complexes of retinal pigment epithelia.Investigative Ophthalmology 12, 354–65.
Iurato, S. (1967) InSubmicroscopic Structure of the Ear (edited byIurato, S.) pp. 80–106. Oxford: Pergamon Press.
Konishi, T. andKelsey, E. (1973) Effect of potassium deficiency on cochlear potentials and cation contents of the endolymph.Acta Otolaryngolica 76, 410–18.
Livingston, R. B., Pfenninger, K., Moor, H. andAkert, K. (1973) Specialized paranodal and interparanodal glial-axonal junctions in the peripheral and central nervous system: A freeze-etching study.Brain Research 58, 1–24.
Mcnutt, N. S. andWeinstein, R. S. (1973) Membrane ultrastructure at mammalian intercellular junctions.Progress in Biophysics and Membrane Biology 26, 45–101.
Raviola, E. andGilula, N. B. (1973) Gap junctions between photo-receptor cells in the vertebrate retina.Proceedings of the National Academy of Sciences USA 70, 1677–81.
Raviola, E. andGilula, N. B. (1975) Intramembrane organization of specialized contacts in the outer plexiform layer of the retina. A freeze-fractured study in monkeys and rabbits.Journal of Cell Biology 65, 192–222.
Reale, E., Luciano, L. andSpitznas, M. (1975) Zonulae occludentes of the myelin lamellae in the nerve fibre layer of the retina and in the optic nerve of the rabbit: A demonstration by the freeze-fracture method.Journal of Neurocytology 4, 131–40.
Reese, T. S. andKarnovsky, M. J. (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase.Journal of Cell Biology 34, 207–17.
Revel, J. P. andKarnovsky, M. J. (1967) Hexagonal array of subunits in intercellular junctions of mouse heart and liver.Journal of Cell Biology 33, C7–12.
Simionescu, M., Simionescu, N. andPalade, G. E. (1975) Segmental differentiations of cell junctions in the vascular endothelium. The microvasculature.Journal of Cell Biology 67, 863–85.
Staehelin, L. A. (1973) Further observations on the fine structure of freeze-cleaved tight junctions.Journal of Cell Science 13, 763–86.
Tice, L. W., Wollman, S. H. andCarter, R. C. (1975) Changes in tight junctions of thyroid epithelium with changes in thyroid activity.Journal of Cell Biology 66, 657–63.
Wade, J. B. andKarnovsky, M. J. (1974) The structure of the zonula occludens. A single fibril model based on freeze-fracture.Journal of Cell Biology 60, 168–80.
Wade, J. B. andKarnovsky, M. J. (1974) Fracture faces of osmotically disrupted zonulae occludentes.Journal of Cell Biology 62, 344–50.
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Gulley, R.L., Reese, T.S. Intercellular junctions in the reticular lamina of the organ of Corti. J Neurocytol 5, 479–507 (1976). https://doi.org/10.1007/BF01181652
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DOI: https://doi.org/10.1007/BF01181652