Abstract
The aim of this study was to investigate the effect of a selective β2-adrenoceptor agonist, clenbuterol, on body composition in tumour-bearing adult and growing mice. Therefore, adult female C57/BL6 mice (n=20) were inoculated subcutaneously with a 3-methylcholanthrene-induced sarcoma and divided into two identical groups. One group received injections twice a day of clenbuterol corresponding to 1 mg/kg body weight, the other group received sham injections. Growing mice (n=20) were similarly divided after tumour inoculation into one study group with clenbuterol injections and one control group. The growing animals were sacrificed on day 11 after commencement of treatment, the adult mice on day 16.
Clenbuterol treatment had no statistically significant effect on accumulated food intake or body composition in the adult mice. However, fooe intake in these animals increased numerically compared to control animals after day 12 of the study. Tumour growth was also unaffected. The growing animals displayed an increased carcass dry weight with borderline significance (p=0.06) and an increased quadriceps muscle fat free dry weight after clenbuterol treatment. Tumour growth was not affected. Food intake measured on a daily basis was significantly increased in the growing clenbuterol treated animals and accumulated food intake was increased with a trend towards statistical significance (p=0.06). The results support the suggestion that treatment with a selective β2-adrenoceptor agonist does not improve body composition in tumour-bearing adult mice relying on spontaneous food intake while growing animals may benefit from such treatment.
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References
Agbenyega, E. T. and Warenham, A. C. (1990) Effect of clenbuterol on normal and denervated muscle growth and contractility.Muscle Nerve 13:199–203.
Baillie, A. G. S. and Garlick, P. J. (1992) Attenuated response of muscle protein synthesis to fasting and insulin in adult female rats.Am. J. Physiol 262:E1-E5.
Brennan, M. F. (1981) Total parenteral nutrition in the cancer patientN. Engl. J. Med. 217:375–382.
Chance, W., Cao, L. and Zhang, F. S. (1991a) Clenbuterol treatment increases muscle mass and protein content of tumor-bearing rats maintained on total parenteral nutrition.J. Parenteral and Enteral. Nutr. 15:530–535.
Chance, W. T., von allmen, D. and Benson, D. (1991b) Clehbuterol decreases catabolism and increases hypermetabolism in burned rats.J. Trauma 31:365–370.
Choo, J. J., Horan, M. A., Little, R. A. and Rothwell, N. J. (1989) Muscle wasting associated with endotoxemia in the rat: modification by the β2-adrenoceptor agonist clenbuterol.Biosci. Rep. 9:615–621.
Choo, J. J., Horan, M. A., Little, R. A. and Rothwell, N. J. (1990) Effects of the β2-adrenoceptor agonist, clenbuterol, on muscle atrophy due to food deprivation in the rat.Metabolism 39:647–650.
Choo, J. J., Horan, M. A. Little, R. A. and Rothwell, N. J. (1992) Anabolic effects of clenbuterol on skeletal muscle are mediated by β2-adrenoceptor activation.Am. J. Physiol. 263:E50-E56.
Drott, C., Svaninger, C. and Lundholm, K. (1988) Increased urinary excretion of cortisol and catecholamines in malnourished cancer patients.Ann. Surg. 208:645–650.
Drott, C., Persson, H. and Lundholm, K. (1989) Cardiovascular and metabolic response to adrenaline infusion in undernourished patients with and without cancer.Clin. Physiol. 9:427–439.
Eadara, J. K., Dalrymple, R. H. and DeLay, R. L. (1989) Effects of cimaterol, a beta-adrenergic agonist, on lipid metabolism in rats.Metabolism 38:522–529.
Edén, E., Lindmark, L., Karlberg, I. and Lundholm K. (1983) Role of whole body lipids and nitrogen as limiting factors for survival in tumor-bearing mice with anorexia and cachexia.Cancer Res. 43:3707–3711.
Edén, E., Edström, S., Bennegård, K., Lindmark, L. and Lundholm, K. (1985) Glycerol dynamics in weight-losing cancer patients.Surgery 97:176–184.
Hill, G. L. (1988) Surgical nutirtion: time for some clinical common sense.Br. J. Surg. 75:729–730.
Klein, S., Simes, J. and Blackburn, G. L. (1986) Total parenteral nutrition and cancer clinical trials.Cancer. 58:1378–1386.
Lundholm, K. (1985) Origins of emaciation of cancer patients.Med. Oncol. and Tumor Pharmacother. 2:183–187.
Rothwell, N. J. and Stock, M. J. (1987) Effect of a selective β2 adrenergic agonist (clenbuterol) on energy balance and body composition in normal and protein-deficient rats.Biosci. Rep. 7:933–940.
Svaninger, G., Bennegård, K., Ekman, L., Ternell, M. and Lundholm, K. (1983) Lack of evidence for elevated breakdown rate of skeletal muscles in weight-losing tumor-bearing mice.J. Natl. Cancer Inst. 71:341–346.
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Hyltander, A., Svaninger, G. & Lundholm, K. The effect of clenbuterol on body composition in spontaneously eating tumour-bearing mice. Biosci Rep 13, 325–331 (1993). https://doi.org/10.1007/BF01150477
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DOI: https://doi.org/10.1007/BF01150477