Summary
A method is presented for the determination of aflatoxins in animal tissues. It consists of extraction of aflatoxins from the sample with methanol, treatment of the residue with a mixture of dimethoxymethane-methanol (4 + 1) to further precipitate proteins, evaporation of dimethoxymethane followed by a liquid-liquid defatting with hexane and heating of the methanol-water mixture before the transfer of aflatoxins into chloroform. Chloroform extracts are further purified by silica gel—acidic alumina—anhydrous sodium sulfate column chromatography.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Van Zytvels, W.A., Kelley, D.C., Dennis, S.M.: Poultry Sci.49, 1350 (1970)
Mintzlaff, H.J., Lötzsch, R., Tauchmann, F., Mayer, W., Leistner, L.: Die Fleischwirtschaft54 (4), 774 (1974)
Armbrecht, B.H., Wiseman, H.G., Shalkop, W.T.: Environ. Physiol.1, 198 (1971)
Krogh, P., Hald, B., Hasselager, E., Madsen, A., Mortensen, H.P., Larsen, A.E., Campbell, A.D.: Offic. J. IUPAC,35, 275 (1973)
Jemmali, M., Murthy, T.R.K.: 2nd Internat. Symposium on Mycotoxins in Foods (July 23–25). Pulawy Poland 1974
McKinney, J.D., Cavanagh, G.G., Bell, J.J., Hoversland, A.S., Nelson, D.M., Pearson, J.M., Selkirk, R.J.: J. Am. Oil Chemists' Soc.50, 99 (1973)
Bullerman, L.B., Hartman, P.A., Ayres, J.C.: JAOAC52, 638 (1969)
Jacobson, W.C., Harmeyer, W.C., Wiseman, H.G.: J. Dairy Sci.54, 21 (1971)
Strzelecki, E.L.: Acta Microbiologica Polonica4, 155 (1972)
Brown, N.L., Nesheim, S., Stack, M.E., Ware, G.: J. Assoc. Offic. Anal. Chemists56, 1437 (1973)
Official Methods of Analysis, 11th Ed. AOAC. Washington D. C. Chapt. 26, 1970
Pons, W.A., Cucullu, A.F., Lee, L.S.: J. Assoc. Offic. Anal. Chemists56, 1431 (1973)
Jemmali, M.: Ann. Biol. Anim. Bioch. Biophys.14 (4-8) 845 (1974)
Schuller, P.L., Verhulsdonk, C.A.H., Paulsch, W.E.: Arzneimittel Forsch.20, 1517 (1970)
Schuller, P.L., Ockhuizen, T.A., Marquarot, P.: Arzneimittel Forsch.17, 888 (1967)
Pohland, A.E., Chishmac, M.E., Andrellos, P.J.: J. Assoc. Offic. Anal. Chemists51, 907 (1968)
Andrellos, P.J., Reid, G.: J. Assoc. Offic. Anal. Chemists47, 802 (1964)
Stack, M.E., Pohland, A.E., Dantzman, D.G., Nesheim, S.: J. Assoc. Offic. Anal. Chemists55, 313 (1972)
McKinney, J.D.: J. Amer. Oil Chemists' Soc.49, 444 (1972)
Murthy, T.R.K., Jemmali, M., Rosset, R.: Z. Lebensm. Unters. Forsch.158, 5 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jemmali, M., Murthy, T.R.K. A chemical assay method for the determination of aflatoxin residues in animal tissues. Z Lebensm Unters Forch 161, 13–17 (1976). https://doi.org/10.1007/BF01145414
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01145414