Summary
Under conditions of iron-deprivationHafnia alvei (Enterobacteriaceae) produces ferrioxamine G as the principal siderophore. Maximum hydroxamate siderophore production occurred at medium iron limitation. The ferrioxamines were extracted, purified by gel filtration and chromatography on silica gel yielding a major and a minor siderophore fraction. The minor siderophore fraction contained three siderophores, among which ferrioxamine E could be identified by HPLC and FAB mass spectrometry. Reductive hydrolysis of the ferrioxamine G fraction yielded succinic acid and a mixture of diaminopentane and diaminobutane, as determined by gas-liquid chromatography and GLC/MS. HPLC and FAB mass spectrometry confirmed that the ferrioxamine G fraction consisted of two different species, G1 and G2, possessing molecular masses of 671 Da and 658 Da respectively.
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Reissbrodt, R., Rabsch, W., Chapeaurouge, A. et al. Isolation and identification of ferrioxamine G and E inHafnia alvei . Biol Metals 3, 54–60 (1990). https://doi.org/10.1007/BF01141179
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DOI: https://doi.org/10.1007/BF01141179