Skip to main content
Log in

Autohydrolysis-explosion as pretreatment for the enzymic saccharification of sunflower seed hulls

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Autohydrolysis of sunflower seed hulls at 200°C for 5 min followed by explosive defibration solubilized >80% of the total hemicellulose (heteroxylan) and 85% of the pectic substances. The remaining residue, which consisted of cellulose (38%), lignin (45%) and residual heteroxylan (7%), was highly susceptible to hydrolysis by cellulases (Trichoderma reesei C-30, Meicelase and Onozuka 3S). Enzymic digests containing 10% (w/v) substrate and 20 FPU/g pretreated hull resulted in sugar yields of >40% within 24h. This yield was increased toca. 70% when digests were supplemented with exogenous β-glucosidase. The method has potential use in the bioconversion of sunflower seed hulls into chemicals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Cancalon, P. (1971). J. Am. Oil Chemists Soc.,48, 629.

    Google Scholar 

  2. Eklund, E., Hatakka, A., Mustranta, A. and Nyberg, P. (1976). European J. Appl. Microbiol.,2, 143.

    Google Scholar 

  3. Fan, L.T., Lee, Y.H. and Gharpuray, M.M. (1982). Adv. Biochem. Eng.,23, 157.

    Google Scholar 

  4. Marchessault, R.H., Coulombe, S., Hanai, T. and Morikawa, H. (1980). Trans. Technical Section, Can. Pulp Paper Assoc.,6, TR 52.

    Google Scholar 

  5. Saddler, J.N., Brownell, H.H., Clermont, L.P. and Levitin, N. (1982). Biotechnol. Bioeng.,24, 1389.

    Google Scholar 

  6. Jurasek, L. (1979). Devel. Ind. Microbiol.,20, 177.

    Google Scholar 

  7. Mamers, H., Yuritta, J.P. and D.N. Menz. (1981). Tappi,64, 93.

    Google Scholar 

  8. Nelson, N. (1944). J. Biol. Chem.,153, 375.

    Google Scholar 

  9. Dekker, R.F.H. and Richards, G.N. (1971). J. Sci. Fd. Agric.,22, 441.

    Google Scholar 

  10. Galambos, J.T. (1967). Anal. Biochem.,19, 119.

    PubMed  Google Scholar 

  11. Blakeney, A.B., Harris, P.J., Henry, R.J. and Stone, B.A. (1982). Carbohyd. Res.,113,in press.

  12. Bland, D.E. and Menshun, M. (1971). Appita,25, 110.

    Google Scholar 

  13. Chua, M.G.S. and Wayman, M. (1979). Can. J. Chem.,57, 1141.

    Google Scholar 

  14. Sternberg, D., Vijayakumer, P. and Reese, E.T. (1977) Can. J. Microbiol.,23, 139.

    PubMed  Google Scholar 

  15. Reese, E.T. and Mandels, M., (1980). Biotechnol. Bioeng.,23, 323.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dekker, R.F.H., Wallis, A.F.A. Autohydrolysis-explosion as pretreatment for the enzymic saccharification of sunflower seed hulls. Biotechnol Lett 5, 311–316 (1983). https://doi.org/10.1007/BF01141131

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01141131

Keywords

Navigation