Abstract
Seven residues implicated as acting directly in substrate binding in yeast hexokinase B have been identified in the crystallographic structure by chemical sequencing. The cysteine which is regarded as a residue critically maintaining the active conformation of yeast hexokinase has been selectively labelled and likewise located in the structure. In some parts of the amino acid sequence predicted from the high-resolution electron density map it is found that alignments of chemically sequenced peptides can be made unambiguously; however, the extent of matching to the predicted sequence varies considerably along the chain.
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Gray, A.J.W., Barron, N.M. & Barnard, E.A. Location in the yeast hexokinase structure of residues related to the enzyme activity. Biosci Rep 3, 963–971 (1983). https://doi.org/10.1007/BF01140666
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DOI: https://doi.org/10.1007/BF01140666