Abstract
Spermatozoa were collected by microaspiration from the corpus epididymidis of a 42-year-old man with secondary obstructive azoospermia and used for in vitro fertilization. At insemination 61% of the spermatozoa were motile, with a motility index of 157. One of five eggs was fertilized and this was subsequently transferred to the patient's wife at the two-cell stage. Ultrasound examination and changing hormone levels confirmed an ongoing pregnancy, which is currently at 30 weeks of gestation. This technique will provide a useful alternative for the management of some infertile men with obstructive azoospermia.
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References
Trounson AO, Wood EC: Extracorporeal fertilization and embryo transfer. Clin Obstet Gynaecol 1981;8:681–713
Holstein AF: Morphologische Studien am Nebenhoden des Menschen.In Zwanglose Abhandlungen aus dem Gebiel der normalen und pathologischen Anatomie, W Bargmann, W Doerr (eds). Stuttgart, Thieme, 1969
World Health Organization: Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. Singapore, Press Concern, 1980
Leeton J, Trounson A, Jessup D, Wood EC: The technique of human embryo transfer. Fertil Steril 1982;38:156–161
Mahadevan MM, Trounson AO: Removal of cumulus around the human oocyte fromin vitro fertilization. Fertil Steril (in press)
Bedford JM: Evolution of the sperm maturation and sperm storage functions of the epididymis.In The Spermatozoon, DW Fawcett, JM Bedford (eds). Baltimore, Urban and Schwarzenberg, 1979, pp 7–21
Orgebin-Crist MC, Danzo BJ, Davies J: Endocrine control of the development and maintenance of sperm fertilizing ability in the epididymis.in Handbook of Physiology: Endocrinology Vol V, RO, Greep, DW Hamilton (eds). Washington, DC, American Physiology Society, 1975, pp 319–338
Bedford JM, Calvin HI, Cooper GW: The maturation of spermatozoa in the human epididymis. J Reprod Fertili 1973, Suppl 18:199–213
Moore HDM, Hartman TD, Pryor JP: Development of the oocyte-penetrating capacity of spermatozoa in the human epididymis. Int J Androl 1983;6:310–318
de Kretser DM, Yates CA, McDonald J, Leeton JF, Southwick GJ, Temple-Smith PD, Trounson AO, Wood EC: The use ofin vitro fertilization in the management of male infertility.In Proceedings of Vth Renier de Graaf Symposium. Amsterdam, Exerpta Medica, (in press)
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Temple-Smith, P.D., Southwick, G.J., Yates, C.A. et al. Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J Assist Reprod Genet 2, 119–122 (1985). https://doi.org/10.1007/BF01131497
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DOI: https://doi.org/10.1007/BF01131497