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Analysis of human spermatozoa before and after processing for in vitro fertilization

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Abstract

Washed incubated spermatozoa processed for in vitro fertilization were compared to the spermatozoa from the same unprocessed ejaculate in 31 randomly selected normal males (Group 1) and 7 subfertile moles (Group 2). In Group I there was a significant increase in motility (P=0.001) as well as a significant increase in normal morphology (P=0.001). All categories of morphologically abnormal sperm decreased, with a significant decrease in those exhibiting small heads (P=0.01), tapered heads (P=0.01), cytoplasmic droplets (P=0.001), bent midpieces (P=0.01), or coiled tails (P=0.02). There was also a significant decrease in immature germ cells (P=0.001) as well as white blood cells (P=0.02). Group 2 also showed a significant increase in motility (P=0.01). and a significant increase in normal morphology (P=0.01). There was a decrease in various categories of abnormal sperm, with a significant decrease in sperm with cytoplasmic droplets (P=0.02).

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Mcdowell, J.S., Veeck, L.L. & Jones, H.W. Analysis of human spermatozoa before and after processing for in vitro fertilization. J Assist Reprod Genet 2, 23–26 (1985). https://doi.org/10.1007/BF01130828

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