Abstract
The binding of doxorubicin to large unilamellar vesicles consisting of cardiolipin or other anionic phospholipids was analyzed in terms of the local drug concentration at the membrane surface, according to the Gouy-Chapman theory. The analysis suggests strong positive binding cooperativity. Part of the drug binds in the uncharged form. The affinity for cardiolipin and other anionic phospholipids is comparable. A binding level of 0.5 doxorubicin per lipid-phosphorus is reached when the local concentration of free doxorubicin monomer-equivalents at the membrane surface is about 0.2–0.7 mM. This contrasts with earlier findings indicating a 300–1000 fold higher affinity for cardiolipin. The present analysis provides an explanation for this apparent discrepancy.
Similar content being viewed by others
References
Capranico, G., Zunino, F., Kohn, K. W. and Pommier, Y. (1990)Biochemistry 29: 562–569.
Griffin, E. A., Vanderkooi, J. M., Maniara, G. and Erecinska, M. (1986)Biochemistry 25:7875–7880.
Escriba, P. V., Ferrer-Montiel, A. V., Ferragut, J. A. and Gonzalez-Ros, J. M. (1990)Biochemistry 29:7275–7282.
Burke, T. G. and Tritton, T. R. (1985)Biochemistry 24:1768–1776.
Nicolay, K., Timmers, R. J. M., Spoelstra, E., van der Neut, R., Fok, J. J., Huigen, Y., Verkleij, A. and de Kruijff, B. (1984)Biochim. Biophys. Acta 778:359–371.
Nicolay, K., Sautereau, A. M., Tocanne, J. F., Brasseur, R., Huart, P., Ruysschaert, J. M. and de Kruijff, B. (1988)Biochim Biophys. Acta 940:197–208.
De Wolf, F. A., Maliepaard, M., van Dorsten, F., Berghuis, I., Nicolay, K. and de Kruijff, B. (1991)Biochim. Biophys. Acta 1096:67–80.
Hannun, Y. A., Loomis, C. R. and Bell, R. M. (1986)J. Biol. Chem. 261:7184–7190.
Nishizuka, Y. (1986)Science 233:305–312.
Nicolay, K. and de Kruijff, B. (1987)Biochim. Biophys. Acta 892:320–330.
Goormaghtigh, E., Chatelain, P., Caspers, J. and Ruysschaert, J. M. (1980)Biochim. Biophys. Acta 597:1–14.
Goormaghtigh, E., Chatelain, P., Caspers, J. and Ruysschaert, J. M. (1980)Biochem. Pharmacol. 29:3003–3010.
Constantinides, P. P., Wang, Y. Y., Burke, T. G. and Tritton, T. R. (1990)Biophys. Chem. 35:259–264.
De Wolf, F. A., Demel, R. A., Bets, D., van Kats, C. and de Kruijff, B. (1991)FEBS Lett. 288:237–240.
Goormaghtigh, E., Huart, P., Praet, M., Brasseur, R. and Ryusschaert, J. M. (1990)Biophys. Chem. 35:247–257.
McLaughlin, S. (1989)Ann. Rev. Biophys. Biophys. Chem. 18:113–136.
Langner, M., Cafiso, D., Marcelja, S. and McLaughlin, S. (1990)Biophys. J. 57:335–349.
Seelig, A., Allegrini, P. R. and Seelig, J. (1988)Biochim. Biophys. Acta 939:267–276.
Bechiavili, G. and Seelig, J. (1990)Biochemistry 29:10995–11000.
Cevc, G. (1990)Biochim. Biophys. Acta 1031:311–382.
Eksborg, S. (1977)J. Pharmaceut. Sci. 67:782–785.
McLennan, I. J., Lenkinski, R. E. and Yanuka, Y. (1985)Can. J. Chem. 63:1233–1238.
Righetti, P. G., Menozzi, M., Gianazza, E. and Valentini, L. (1979)FEBS Lett. 101:51–55.
Van Deenen, L. L. M. and de Haas, G. H. (1964)Adv. Lip. Res. 2:168–229.
Comfurius, P. and Zwaal, R. F. A. (1977)Biochim. Biophys. Acta 488:36–42.
Smaal, E. B., Romijn, D., Geurts van Kessel, W. S. M., de Bruijff, B. and de Gier, J. (1985)J. Lip Res. 26:633–637.
Rouser, G., Fleischer, S. and Yamamoto, A. (1970)Lipids 5:494–496.
Demel, R. A., Geurts van Kessel, W. S. M., Zwaal, R. F. A., Roelofsen, B. and van Deenen, L. L. M. (1975)Biochim. Biophys. Acata 406:97–107.
Henry, N., Fantine, E. O., Bolard, J. and Garnier-Suillerot, A. (1985)Biochemistry 24:7085–7092.
Chaires, J. B., Dattagupta, N. and Crothers, D. M. (1982)Biochemistry 21:3927–3932.
Frézard, F. and Garnier-Suillerot, A. (1990)Biochim. Biophys. Acta 1036:121–127.
Sturgeon, R. J. and Schulman, S. G. (1977)J. Pharmaceut. Sci. 66:958–961.
Cheneval, D., Müller, M., Toni, R., Ruetz, S. and Carafoli, E. (1985)J. Biol. Chem. 260:13003–13007.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Wolf, F.A. Binding of doxorubicin to cardiolipin as compared to other anionic phospholipids—An evaluation of electrostatic effects. Biosci Rep 11, 275–284 (1991). https://doi.org/10.1007/BF01127503
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01127503