Abstract
The specificity of action of antisense RNA for one ofDrosophila low molecular weight heat shock proteins (hsp 23) was tested at the translational level using the rabbit reticulocyte lysate cell-free system. T7 polymerase-driven transcripts of hsp 23 in the antisense orientation were mixed with mRNA from heat-shocked cells under various stringency conditions prior to translationin vitro. Although the four small hsps show considerable sequence homology in their coding sequences, antisense hsp 23 RNA was shown to specifically inhibit hsp 23 mRNA translation under both high (formamide, 45°C and low stringency (37°C conditions. This suggests that the 5′ leader and the ribosome binding region of mRNA are of prime importance in the specificity of action of antisense RNA at the translational level.
References
Lindquist, S. (1986).Ann. Rev. Biochem. 55:1151–1191.
Craig, E. A. (1985).CRC Crit. Rev. Biochem. 18:239–280.
Melton, D. A. (1985).Proc. Natl. Acad. Sci. USA 82:144–148.
Izant, J. G. and Weintraub, H. (1984).Cell 36:1007–1015.
Rosenberg, U. R., Preiss, A., Seifert, E., Jäckle, H. and Knipple, D. C. (1985).Nature (London) 313:703–706.
McGarry, T. J. and Lindquist, S. (1986).Proc. Natl. Acad. Sci. USA 83:399–403.
Corces, V., Holmgren, R., Freund, R., Morimoto, R. and Meselson, M. (1980).Proc. Natl. Acad. Sci. USA 77:5390–5393.
Ingolia, T. D. and Craig, E. A. (1982).Proc. Natl. Acad. Sci. USA 79:2360–2364.
Southgate, R., Ayme, A. and Voellmy, R. (1983).J. Mol. Biol. 165:35–57.
Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982). In:Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor.
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K. and Green, M. R. (1984).Nucl. Acid. Res. 12:7035–7056.
Kelley, P. M. and Schlesinger, M. J., (1982).Mol. Cell. Biol. 2:267–274.
Pelletier, J. and Sonenberg, N. (1985).Cell. 40:515–526.
Chirgwin, J. M., Przybyla, A. F., MacDonald, R. J. and Rutter, W. J. (1979).Biochemistry 18:5294–5299.
Glisin, V., Crkvenjakov, R. and Byus, C. (1974).Biochemistry 13:2633–2637.
Thomas, J. O. and Kornberg, R. D. (1975).Proc. Natl. Acad. Sci. USA 72:2626–2630.
Laskey, R. and Mills, A. (1975).Eur. J. Biochem. 56:335–341.
Parker, P. J., Coussens, L., Totty, N., Rhee, L., Young, S., Chen, E., Stabel, S., Waterfield, M. D. and Ullrich, A. (1986).Science 233:853–859.
Harland, R. and Weintraub, H. (1985).J. Cell Biol. 101:1094–1099.
Blake, K. R., Murakami, A. and Miller, P. S. (1985).Biochemistry 24:6132–6138.
Cazenave, C., Loreau, N., Toulmé, J.-J. and Hélène, C. (1986),Biochimie 68:1063–1069.
Shakin, S. H. and Liebhaber, S. A. (1986).J. Biol. Chem. 261:16018–16025.
Kozak, M. (1986).Proc. Natl. Acad. Sci. USA 83:2850–2854.
Leinwand, L. and Ruddle, F. H. (1977).Science 197:381–383.
Payvar, F. and Schimke, R. T. (1979).J. Biol. Chem. 254:7636–7642.
Mortensen, R. M., Rachubinski, R. A., Fujiki, Y. and Lazarow, P. B. (1984).Biochem. J. 223:547–550.
Mezl, V. A., Clark, J. and Garber, P. M. (1986).Biochem. Cell Biol. 64:504–508.
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Nicole, L.M., Tanguay, R.M. On the specificity of antisense RNA to arrestin vitro translation of mRNA coding forDrosophila hsp 23. Biosci Rep 7, 239–246 (1987). https://doi.org/10.1007/BF01124795
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DOI: https://doi.org/10.1007/BF01124795