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Direct radioactive labelling of poly(ADP-ribose) in developingXenopus laevis embryos

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Bioscience Reports

Abstract

It has not previously been possible to label the nuclear protein modification poly(ADP-ribose) directly from NAD because of the impermeability of the cell membrane. We have overcome this important problem by micro-injection of radioactively labelled NAD intoxenopus laevis early embryos. The polymer was identified and then quantified by its insensitivity to DNAase, RNAase, and spleen phosphodiesterase and by the chromatographic mobility of the products of digestion with snake-venom phosphodiesterase. The quantity of poly(ADP-ribose) present after 25 h of development (129 ng/mg DNA) is lower than that found in fully differentiated tissue.

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References

  • Benjamin BC & Gill DM (1980) J. Biol. Chem.255, 10502–10508.

    PubMed  Google Scholar 

  • Berger NA, Weber G, Kaichi AS & Petzold SJ (1978) Biochim. Biophys. Acta519, 105–117.

    PubMed  Google Scholar 

  • Caplan AI & Rosenberg MJ (1975) Proc. Natl. Acad. Sci. U.S.A.72, 1852–1857.

    PubMed  Google Scholar 

  • Durkacz BW, Omidji O, Gray DA & Shall S (1980) Nature283, 593–596.

    PubMed  Google Scholar 

  • Farzaneh F, Zalin R & Shall S (1980) in ‘Novel ADP-Ribosylation of Regulatory Enzymes and Proteins’ (Smulson M & Sugimura T, eds) Elsevier, London, pp 217–225.

    Google Scholar 

  • Farzaneh F, Zalin R, Brill D & Shall S (1982) Nature300, 362–366.

    PubMed  Google Scholar 

  • Ferro AM, Minaga T, Piper WN & Kun E (1978) Biochim. Biophys. Acta519, 291–305.

    PubMed  Google Scholar 

  • Gurdon JB (1967) in ‘Techniques in Developmental Biology’ (Wessels NK & Wilt F, eds), pp 75–84, Thomas Cromwell.

  • Halldorsson H, Gray DA & Shall S (1978) FEBS Lett.85, 349–352.

    PubMed  Google Scholar 

  • Hilz H (1981) Hoppe-Seyler's Z. Physiol. Chem.362, 1415–1425.

    PubMed  Google Scholar 

  • Hilz H & Stone P (1976) Rev. Physiol. Biochem. Pharmacol.76 1–58.

    PubMed  Google Scholar 

  • Jacobson MK, Levi V, Juarez-Salinas H, Barton RA & Jacobson EL (1980) Cancer Res.40, 1797–1802.

    PubMed  Google Scholar 

  • Johnstone AP & Williams GT (1982) Nature300, 368–370.

    PubMed  Google Scholar 

  • Mandel P, Okazaki H & Niedergang C (1982) Prog. Nucl. Acid Res. Mol. Biol.27, 1–51.

    Google Scholar 

  • Nduka N, Skidmore CJ & Shall S (1980) Eur. J. Biochem.105, 525–530.

    PubMed  Google Scholar 

  • Purnell MR, Stone PR & Whish WJD (1980) Biochem. Soc. Trans.8, 215–227.

    PubMed  Google Scholar 

  • Rastl E & Swetly P (1978) J. Biol. Chem.253, 4333–4340.

    PubMed  Google Scholar 

  • Skidmore CJ, Davies MI, Goodwin PM, Halldorsson H, Lewis PJ, Shall S & Zia'ee A-A (1979) Eur. J. Biochem.101, 135–145.

    PubMed  Google Scholar 

  • Whish WJD, Davies MI & Shall S (1975) Biochem. Biophys. Res. Commun.65, 722–730.

    PubMed  Google Scholar 

  • Williams GT (1979) D. Phil. Thesis, University of Sussex.

  • Williams GT (1983) J. Cell. Biochem. supp.7A, 16.

    Google Scholar 

  • Williams GT, Shall S & Ford CC (1983) Biochim. Biophys. Acta762, 272–280.

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Cell and Molecular Biology Laboratory, University of Sussex, BN1 9QG, Brighton, Sussex, UK

    Gwyn T. Williams & Sydney Shall

  2. Department of Immunology, St. George's Hospital Medical School, Cranmer Terrace, SW17 ORE, London, UK

    Christoper C. Ford

Authors
  1. Gwyn T. Williams
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  2. Sydney Shall
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  3. Christoper C. Ford
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Williams, G.T., Shall, S. & Ford, C.C. Direct radioactive labelling of poly(ADP-ribose) in developingXenopus laevis embryos. Biosci Rep 3, 461–467 (1983). https://doi.org/10.1007/BF01121957

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  • DOI: https://doi.org/10.1007/BF01121957

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