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A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains

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Bioscience Reports

Abstract

A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fucα1→2Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure:

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References

  1. Köhler G & Milstein C (1975) Nature256, 495–497.

    Google Scholar 

  2. Feizi T, Kabat EA, Vicari G, Anderson B & Marsh WL (1971) J. Immunol.106, 1578–1592.

    Google Scholar 

  3. Niemann H, Watanabe K, Kakomori S, Childs RA & Feizi T (1978) Biochem. Biophys. Res. Commun.81, 1286–1293.

    Google Scholar 

  4. Watanabe K, Hakomori S, Childs RA & Feizi T (1979) J. Biol. Chem.254, 3221–3228.

    Google Scholar 

  5. Gooi HC, Feizi T, Kapadia A, Knowles BB, Solter D & Evans MJ (1981) Nature292, 292, 156–158.

    Google Scholar 

  6. Brockhaus M, Magnani JL, Blaszczyk M, Steplewski Z, Koprowski H, Karlsson K-A, Larson G & Ginsburg V (1981) J. Biol. Chem.256, 13223–13225

    Google Scholar 

  7. Anger BR, Lloyd KO, Oettgen HF & Old LJ (1982) Hybridoma1, 139–147.

    Google Scholar 

  8. Brockhaus M, Magnani JL, Herlyn M, Blaszczyk M, Steplewski Z, Koprowski H & Ginsburg V (1982) Arch. Biochem. Biophys.217, 647–651.

    Google Scholar 

  9. Gooi HC, Thorpe SJ, Hounsell EF, Rumpold H, Kraft D, Förster O & Feizi T (1983) Eur. J. Immunol., in press.

  10. Hakomori S, Nudelman E, Kannagi R & Levery SB (1982) Biochem. Biophys. Res. Commun.109, 36–44.

    Google Scholar 

  11. Feizi T (1983) Biochem. Soc. Trans.11, 263–266.

    Google Scholar 

  12. Gooi HC, Williams LK, Uemura K, Hounsell EF, McIlhinney RAJ & Feizi T, Molec. Immunol., in press.

  13. Hakomori S, Nudleman E, Levery S, Solter D & Knowles BB (1981) Biochem. Biophys. Res. Commun.100, 1578–1586.

    Google Scholar 

  14. Willison KR & Stern PL (1978) Cell14, 785–793.

    Google Scholar 

  15. Magnani J, Nillson B, Brockhaus M, Zopf D, Steplewski Z, Koprowski H & Ginsburg V (1982) Fed. Proc.41, 898.

    Google Scholar 

  16. Pukel CS, Lloyd KO, Travassos LR, Dippold WG, Oettgen HF & Old LJ (1982) J. Exp. Med.155, 1133–1147.

    Google Scholar 

  17. Willison KR, Karol RA, Suzuki A, Kundu SK & Marcus DM (1982) J. Immunol.129, 603–609.

    Google Scholar 

  18. Baldwin RW, Harris JR & Price MR (1973) Int. J. Cancer11, 385–397.

    Google Scholar 

  19. Galfré G, Howe SC, Milstein C, Butcher GW & Howard JC (1977) Nature266, 550–552.

    Google Scholar 

  20. Picard J, Waldron-Edward D & Feizi T (1978) J. Clin. Lab. Immunol.1, 119–128.

    Google Scholar 

  21. Watkins WM (1980) inAdvances in Human Genetics, vol. 10, (Harris H & Hirschhorn K, eds), pp 1–136 and 379–385, Plenum Publishing Co.

  22. Lloyd KO & Kabat EA (1968) Proc. Natl. Acad. Sci. U.S.A.61, 1470–1477.

    Google Scholar 

  23. Johnson PH, Yates AD & Watkins WM (1981) Biochem. Biophys. Res. Commun.100, 1611–1618.

    Google Scholar 

  24. Prieels J-P, Monnom D, Dolmans M, Beyer TA & Hill RL (1981) J. Biol. Chem.256, 10456–10463.

    Google Scholar 

  25. Hakomori S & Kobata A (1974) inThe Antigens, vol 2 (Sela M, ed), pp 79–140, Academic Press, New York.

    Google Scholar 

  26. Smith EL, McKibbin JM, Karlsson K-A, Pascher I & Samuelsson BE (1975) Biochim. Biophys. Acta388, 171–179.

    Google Scholar 

  27. Karlsson K-A & Larson G (1981) J. Biol. Chem.256, 3512–3524.

    Google Scholar 

  28. Kannagi R, Nudelman E, Levery SB & Hakomori S (1982) J. Biol. Chem.257, 14865–14874.

    Google Scholar 

  29. Hallgren P & Lunblad A (1977) J. Biol. Chem.252, 1023–1033.

    Google Scholar 

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Authors and Affiliations

  1. Cancer Research Campaign Laboratories, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    A. Brown, M. J. Embleton & R. W. Baldwin

  2. Applied Immunochemistry Research Group, Clinical Research Centre, Watford Road, HAI 3UJ, Harrow, Middlesex, UK

    T. Feizi, H. C. Gooi & J. K. Picard

Authors
  1. A. Brown
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  2. T. Feizi
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  3. H. C. Gooi
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  4. M. J. Embleton
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  5. J. K. Picard
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  6. R. W. Baldwin
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Brown, A., Feizi, T., Gooi, H.C. et al. A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains. Biosci Rep 3, 163–170 (1983). https://doi.org/10.1007/BF01121947

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  • DOI: https://doi.org/10.1007/BF01121947

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