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Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes

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Abstract

An increase in bisphosphoglycerate mutase (BPGM) and a decrease in pyruvate kinase (PK), i.e. a decrease in PK/BPGM ratio, was observed in red cell populations from anemic rats containing 95% down to 3% reticulocytes in blood. Such a ratio has been used to study the fractionation of recticulocytes, according to their degree of maturation, after counter-current distribution of those cell populations in dextrahpoly (ethylene glycol) two-phase systems. When applying this procedure to the fractionation according to age of erythrocytes from normal rats, the decrease of PK with cellular age was observed without a significant variation in BPGM activity.

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Authors and Affiliations

  1. Departamento de Bioquimica y Biologia Molecular, Universidad de Alcalã de Henares, Madrid, Spain

    J. Luque, M. D. Delgado, P. Rodriguez-Horche, M. T. Company & M. Pinilla

Authors
  1. J. Luque
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  2. M. D. Delgado
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  3. P. Rodriguez-Horche
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  4. M. T. Company
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  5. M. Pinilla
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Luque, J., Delgado, M.D., Rodriguez-Horche, P. et al. Bisphosphoglycerate mutase and pyruvate kinase activities during maturation of reticulocytes and ageing of erythrocytes. Biosci Rep 7, 113–119 (1987). https://doi.org/10.1007/BF01121874

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  • DOI: https://doi.org/10.1007/BF01121874

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