Abstract
There is no shift of emission maximum (F470nm) of bovine serum albumin (BSA)-l-anilino-8-naphthatene sulphonic acid (ANS) complex in the pesence of delta-9-tetrahydrocannabinol (delta-9-THC) alone and cannabidiol (CBD) or cannabinol (CBN) in the presence and absence of delta-9-THC. Delta-9-THC (1.66–13.33 μM) and CBD at higher concentrations (13.33–20.0 μM) produce a concentration-dependent significant quenching of fluorescence of BSA-ANS complex, but CBN (l.66–20.0 μM) as well as CBD at lower concentrations (1.66–6.66 μM) fails to produce any significant change in Iluorescence intensity under similar conditions. Furthermore, neither CBD nor CBN is able to affect the delta-9-THC-induced quenching of fluorescence intensity of BSA-ANS complex. These results indicate that the binding of cannabinoids to the ANS binding sites of BSA molecule are in the order detta-9-THC > CBr3 > CBN, and CBD or CBN does not have any influence on the binding of delta-9-THC to BSA molecules under these conditions.
Similar content being viewed by others
References
Brocklehurst JR, Freedman RB, Hancock DJ & Radda GK (1970) Biochem. J.116, 721–731.
Haque SJ & Poddar MK (1983) IRCS Med. Sci.11, 290–291.
Haque SJ, Ray SK & Poddar MK (1983) Indian J. Biochem. Biophys. (in Press).
Hollister LE (1970) Nature (London)227, 968–969.
Jones G & Pertwee RG (1972) Brit. J. Pharmacol.45, 375–377.
Karniol IG & Carlini EA (1972) J. Pharm. Pharmac.24, 833–835.
Mechoulam R (1970) Science N Y168, 1159–1166.
Paton WDM & Pertwee RG (1972) Brit. J. Pharmacol.44, 250–261.
Poddar MK & Ghosh JJ (1972) Sci. & Cult.38, 377–378.
Poddar MK, Bhattacharyya KC & Ghosh JJ (1974) Biochem. Pharmacol.23, 758–759.
Poddar MK, Ghosh JJ & Datta J (1975) Sci. & Cult.41, 492–495.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Haque, S.J., Poddar, M.K. Interactions of cannabinoids with bovine serum albumin. Biosci Rep 4, 239–243 (1984). https://doi.org/10.1007/BF01119659
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01119659