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DNAse I—actin complex: An immunological study

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Bioscience Reports

Abstract

DNAse I - actin complex formation is studied in the presence of different anti actin antibody populations. The binding of DNAse I to actin is shown to be affected by antibodies specific to a central region in actin sequence (168–226). The C- and N-extremities of actin are shown to be in spatial proximity at the surface of the actin monomer and far from the binding area of DNAse I.

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References

  1. Craig SW & Pollard TD (1982) TIBS7, 88–92.

    Google Scholar 

  2. Carlsson L, Nystrom LE, Sundkist I, Markey F & Lindberg U (1977) J. Mol. Biol.115, 465–483.

    PubMed  Google Scholar 

  3. Reichtein E & Korn ED (1979) J. Biol. Chem.254, 6174–6179.

    PubMed  Google Scholar 

  4. Fattoum A, Roustan C, Feinberg J & Pradel LA (1980) FEBS Lett.118, 237–240.

    PubMed  Google Scholar 

  5. Rohr G & Mannherz HG (1978) Eur. J. Biochem.89, 151–157.

    PubMed  Google Scholar 

  6. Lazarides E & Linberg U (1974) Proc. Natl. Acad. Sci. U.S.A.71, 4742–4746.

    PubMed  Google Scholar 

  7. Mannherz HG, Barrington Leigh J, Leberman R & Pfang H (1975) FEBS Lett.60, 34–38.

    PubMed  Google Scholar 

  8. Suck D, Kabsch W & Mannherz HG (1981) Proc. Natl. Acad. Sci. U.S.A.78, 4319–4323.

    PubMed  Google Scholar 

  9. De Couet HG (1983) J. Musc. Res. Cell Motility4, 405–427.

    Google Scholar 

  10. Sutoh K (1984) Biochemistry23, 1942–1946.

    PubMed  Google Scholar 

  11. Spudich JA & Watt S (1971) J. Biol. Chem.246, 4866–4871.

    PubMed  Google Scholar 

  12. Rich SA & Estes JE (1976) J. Mol. Biol.104, 777–792.

    PubMed  Google Scholar 

  13. Callahan HJ, Liberti PA & Maurer PH (1974) Immunochemistry11, 197–202.

    PubMed  Google Scholar 

  14. Takashi R (1979) Biochemistry18, 5164–5169.

    PubMed  Google Scholar 

  15. Nakamura T, Yamaguchi M & Yanagisawa T (1979) J. Biochem.85, 627–631.

    PubMed  Google Scholar 

  16. Johnson, P, Wester PJ & Hikida RS (1979) Biochim. Biophys. Acta578, 253–257.

    PubMed  Google Scholar 

  17. Benyamin Y, Roustan C & Boyer M (1983) FEBS Lett.160, 41–45.

    PubMed  Google Scholar 

  18. Roustan C, Benyamin Y & Boyer M (1984) J. Muscle Res. Cell Motil. (in press).

  19. Jacobson GR & Rosenbusch JP (1976) Proc. Natl. Acad. Sci. U.S.A.73, 2742–2746.

    PubMed  Google Scholar 

  20. Laemmli UK (1970) Nature (London)227, 680–685.

    Google Scholar 

  21. Bottomley RC & Trayer TP (1975) Biochem. J.149, 365–379.

    PubMed  Google Scholar 

  22. Bulinski JC, Kumar S, Titani K & Hauschka SD (1983) Proc. Natl. Acad. Sci. U.S.A.80, 1506–1510.

    PubMed  Google Scholar 

  23. Hudson EN & Weber G (1973) Biochemistry12, 4154–4160.

    PubMed  Google Scholar 

  24. Benyamin Y, Robin Y and Thoaï NV (1973) Eur. J. Biochem.37, 459–466.

    PubMed  Google Scholar 

  25. Langone JJ (1978) J. Immunol. Methods24, 269–285.

    PubMed  Google Scholar 

  26. Kunitz MJ (1950) J. Gen. Physiol.33, 349–362.

    PubMed  Google Scholar 

  27. Vandekerckhove J, De Couet HG & Weber K (1983) in: Actin: Structure and Function in Muscle and Non Muscle Cells (Dos Remedios C & Barden J, eds), pp 241–248, Academic Press, New York.

    Google Scholar 

  28. Mannherz HG, Goody RS, Konrad M & Novak E (1980) Eur. J. Biochem.104, 367–379.

    PubMed  Google Scholar 

  29. Ikkai T, Mihashi K & Kouyama T (1980) FEBS Lett109, 216–218.

    PubMed  Google Scholar 

  30. Ng JSY & Burtnick LD (1982) Int. J. Macromol.4, 215–218.

    Google Scholar 

  31. Mannherz HG, Dieckhoff J, Suck D, Kabsch W, Kriebel K & Frimer M (1984) 13th European Conference on Muscle and Motility (Switzerland), Abstract no. 53, p. 29.

  32. Muszbek L & Laki K (1974) Proc. Natl. Acad. Sci. U.S.A.71, 2208–2211.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Centre de Recherches de Biochimie Macromoléculaire du C.N.R.S., BP 5051, F-34033, Montpellier Cedex, France

    M. Boyer & C. Roustan

  2. Laboratoire de Biochimie et Ecologie des Invertébrés Marins de l'Ecole Pratique des Hautes Etudes, BP 5051, F-34033, Montpellier Cedex, France

    Y. Benyamin

Authors
  1. M. Boyer
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  2. C. Roustan
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  3. Y. Benyamin
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Boyer, M., Roustan, C. & Benyamin, Y. DNAse I—actin complex: An immunological study. Biosci Rep 5, 39–46 (1985). https://doi.org/10.1007/BF01117439

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  • DOI: https://doi.org/10.1007/BF01117439

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