Skip to main content
SpringerLink
Log in

The amino acid sequence of human sperm protamine P1

  • Published:
Bioscience Reports

Abstract

Human sperm protamines have been extracted from spermatozoa pooled from several donors, converted to their S-pyridylethylated derivatives and resolved into two major components, P1 and PI, by Bio-Rex 70 chromatography. Protamine P1 was further purified by Bio-Gel P-10 chromatography and sequenced directly on a gas phase protein sequencer for 43 residues. To complete the sequence, P1 was cleaved at methionine 36 and the C-terminal tetradecapeptide was purified by h.p.i.c , and sequenced completely. The 50 residue sequence is: 10 20 30 40 ARYRC CRSQS RSRYY RQRQR SRRRR RRSCQ TRRRA MRCCR 50 PRYRP RCRRH. This sequence has a calculated molecular weight of 6674 and is homologous with four other published mammalian protamine sequences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Balhorn R, Gledhill BL & Wyrobek AJ (1977) Biochem.16, 4074–4080.

    Google Scholar 

  • Bellvé AR, Anderson E & Hanley-Bowdoin L (1975) Dev. Biol.47, 349–365.

    Google Scholar 

  • Bloch DP (1969) Genetics61, Suppl. 1, 93–111.

    Google Scholar 

  • Coelingh JP, Pozijn TH & Monfoort CH (1969) Biochim. Biophys. Acta188, 353–356.

    Google Scholar 

  • Coelingh JP, Monfoort CH, Rozijn TH, Leuven JAG, Schiphof R, Steyn-Parve EP, Braunitzer G, Schrank B & Ruhfus A (1972) Biochim. Biophys. Acta285, 1–14.

    Google Scholar 

  • Dayhoff MO, Eck RV & Park CM (1972) In: Atlas of Protein Sequence and Structure (Dayhoff MO, ed), Washington, National Biomedical Research Foudation.

    Google Scholar 

  • Dixon GH, Aiken JM, Jankowski JM, McKenzie DI, Moir R & States JC (1985) Proc. Advanced Study Institute, Sitges, Spain, in press.

  • Gaastra W, Hofstra JL & Kolk AHJ (1978) Biochem. Genetics16, 525–529.

    Google Scholar 

  • IUPAC — ‘A One Letter Notation for Amino Acid Sequeces’ (1968) Eur. J. Biochem5, 151–153. A=Alanine, C=Cysteine, F=Phenylalanine, G=Glycine, H=Histidine, I=Isoleucine, K=Lysine, L=Leucine, M=Methionine, P=Proline, Q=Glutamine, R=Arginine, S=Serine, T=Threonine, V=Valine, and Y=Tyrosine.

    Google Scholar 

  • Kleene KC, Distel RJ & Hecht NB (1985) Biochemistry24, 719–722.

    Google Scholar 

  • Kolk AHJ & Samuel T (1975) Biochim. Biophys. Acta393, 307–319.

    Google Scholar 

  • Monfoort CH, Schiphof R, Rozijn TH & Steyn-Parve EP (1973) Biochim. Biophys. Acta322, 173–177.

    Google Scholar 

  • Pongsawasdi P & Svasti J (1976) Biochim. Biophys. Acta434, 462–473.

    Google Scholar 

  • Sautiere P, Belaiche D, Martinage A & Loir M (1984) Eur. J. Biochem.144, 121–125.

    Google Scholar 

  • Svasti J & Talupphet N (1979) Biochim. Biophys. Acta577, 221–225.

    Google Scholar 

  • Tobita T, Tsutsumi H, Kato A, Suzuki H, Nomoto M, Nakano M & Ando T (1983) Biochim. Biophys. Acta744, 141–146.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Health Sciences Centre, 3330 Hospital Drive N. W., T2N 4N1, Calgary, Alberta, Canada

    D. J. McKay, B. S. Renaux & G. H. Dixon

Authors
  1. D. J. McKay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. S. Renaux
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. H. Dixon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

McKay, D.J., Renaux, B.S. & Dixon, G.H. The amino acid sequence of human sperm protamine P1. Biosci Rep 5, 383–391 (1985). https://doi.org/10.1007/BF01116555

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01116555

Keywords

Search

Navigation