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Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens

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Bioscience Reports

Abstract

A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and highmol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen -{α1(V)}2α2(V) and {α1(V)2α2(V)α3(V)}—which have been separated on DEAE-cellulose. Uterus however appears to be the richest source of both HMW aggregates and the {α1(V)2α2(V)α3(V)} collagen, and a probable relationship between these collagens is discussed.

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Authors and Affiliations

  1. Department of Rheumatology, University of Manchester Medical School, M13 9PT, Manchester, U.K.

    M. Z. Abedin, Shirley Ayad & Jacqueline B. Weiss

Authors
  1. M. Z. Abedin
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  2. Shirley Ayad
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  3. Jacqueline B. Weiss
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Abedin, M.Z., Ayad, S. & Weiss, J.B. Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens. Biosci Rep 2, 493–502 (1982). https://doi.org/10.1007/BF01115247

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  • DOI: https://doi.org/10.1007/BF01115247

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