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Immobilization of enzymes on alumina by means of pyridoxal 5′-phosphate

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Abstract

A number of proteases have been immobilized on alumina in a two-step procedure: the first step converted them into semisynthetic phosphoproteins which, in the second step, spontaneously bonded to alumina through their phosphate function. The immobilized enzymes thus obtained showed the physical properties typical of the inorganic carrier and a high activity on low molecular weight substrates.

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Authors and Affiliations

  1. INSERM (U 58), 60 rue de Navacelles, 34090, MONTPELLIER, France

    M. Pugnière, C. San Juan, M-A. Coletti-Previero & A. Previero

Authors
  1. M. Pugnière
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  2. C. San Juan
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  3. M-A. Coletti-Previero
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  4. A. Previero
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Pugnière, M., San Juan, C., Coletti-Previero, MA. et al. Immobilization of enzymes on alumina by means of pyridoxal 5′-phosphate. Biosci Rep 8, 263–269 (1988). https://doi.org/10.1007/BF01115043

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  • DOI: https://doi.org/10.1007/BF01115043

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