Skip to main content
Log in

Monoclonal antibodies to mammalian neurofilaments

  • Published:
Bioscience Reports

Abstract

Hybrid cell lines secreting monoclonal antibodies against mammalian neurofilaments have been prepared. An improved protocol for the preparation of neurofilaments and methods for the identification and isolation of such hybridomas are presented. The antibodies produced specifically stain only neuronal cell types in both cerebellar sections and culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Anderton BH (1981)J. Muscle Res. and Cell Mo tility, in press.

  • Anderton BH, Ayers M, & Thorpe RC (1978)FEBS Letters 96, 159–163.

    PubMed  Google Scholar 

  • Anderton BH, Thorpe RC, Cohen J, Selvendran S, & Woodhams P (1980)J. Neurocytol. 9, 835–844.

    PubMed  Google Scholar 

  • Bennett GS, Fellini SA, Croop JM, Otto JJ, Bryan J, & Holtzer H (1978a)Proc. Natl. Acad. Sci. U.S.A. 75, 4364–4368.

    PubMed  Google Scholar 

  • Bennett GS, Fellini SA, & Holtzer H (1978b)Differentiation 12, 71–82.

    PubMed  Google Scholar 

  • Blose SH, Shelanski M, & Chacko S (1977)Proc. Natl. Acad. Sci. U.S.A. 74, 662–665.

    PubMed  Google Scholar 

  • Brown JP, Temerius JD, & Hellstrom I (1979)J. Imm. Methods 31, 201–209.

    Google Scholar 

  • Dahl D (1980)FEBS Letters 111, 152–156.

    PubMed  Google Scholar 

  • Fellini SA, Bennett GS, Toyama Y, & Holtzer H (1978)Differentiation 12, 59–69.

    PubMed  Google Scholar 

  • Hoffman PN & Lasek RJ (1975)J. Cell. Biol. 66, 351–366.

    PubMed  Google Scholar 

  • Hudson L & Hay FC (1980)Practical Immunology, Blackwells, London.

    Google Scholar 

  • Jacobs M, Choo F, & Thomas C (1980)Cell. Biol. Int. Rep. 4, 778.

    Google Scholar 

  • Laemmli UK (1970)Nature (Lond.) 227, 680–685.

    Google Scholar 

  • Lazarides E (1980)Nature (Lond.) 283, 249–256.

    Google Scholar 

  • Liem RKH, Yen SH, Salomon GD, & Shelanski ML (1978)J. Cell Biol. 79, 637–645.

    PubMed  Google Scholar 

  • Micko S & Schlaepfer WW (1978)J. Neurochem. 30, 1041–1049.

    PubMed  Google Scholar 

  • Milstein C (1980)Scientific American 243, 56–106.

    Google Scholar 

  • Thorpe RC, Delacourte A, & Anderton BH (1979a)FEBS Letters 181, 275–284.

    Google Scholar 

  • Thorpe RC, Delacourte A, Ayers M, Bullock C, & Anderton BH (1979b)Biochem J. 181, 275–284.

    PubMed  Google Scholar 

  • Wang C, Asai DJ, & Lazarides E (1980)Proc. Natl. Acad. Sci. U.S.A. 77, 1541–1545.

    PubMed  Google Scholar 

  • Wood JN, Anderton BH, & Thorpe RC (1980)Biochem. Soc. Trans. 8, 615–616.

    PubMed  Google Scholar 

  • Yen SH & Fields KL (1981)J. Cell. Biol. 88, 115–126.

    PubMed  Google Scholar 

  • Yen SH, Dahl D, Schachner M, & Shelanski MC (1976)Proc. Natl. Acad. Sci. U.S.A. 73, 529–533.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wood, J.N., Anderton, B.H. Monoclonal antibodies to mammalian neurofilaments. Biosci Rep 1, 263–268 (1981). https://doi.org/10.1007/BF01114913

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01114913

Keywords

Navigation