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Complex-type carbohydrates of apolipoprotein-B of human plasma low-density lipoproteins

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Abstract

Two fractions of glycopeptides containingN-glycosidic asparagine-linked glycans were isolated by concanavalin-A-Sepharose affinity chromatography from Pronase digests of apolipoprotein-B of human low-density lipoproteins. Methylation analysis indicated that the non-binding fraction contains about 0.5 mol complex-type tri- or tetra-antennary oligosaccharides per mol lipoprotein. Structures containing a bisectingN-acetylglucosamine were also detected in this fraction. The weakly binding fraction from the chromatography contains the majority of the complex oligosaccharides of apolipoprotein-B, i.e. 5–6 mol of bi-antennary chains of the transferrin-type per mol of lipoprotein. In addition to sialylated structures, branches containing a terminalN-acetylglucosamine residue were detected on the complex-type glycans of apolipoprotein-B.

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Abbreviations

Apo-B:

apolipoprotein B

LDL:

low-density lipoprotein

References

  1. Kane JP, Hardman DA, Paulus HE (1980) Proc Natl Acad Sci USA 77:2465–69.

    PubMed  Google Scholar 

  2. Goldstein JL, Brown MS (1977) Annu Rev Biochem 46:897–930.

    PubMed  Google Scholar 

  3. Brown MS, Goldstein JL (1983) J Clin Invest 72:743–47.

    PubMed  Google Scholar 

  4. Ehnholm C, Garoff H, Renkonen O, Simons K (1972) Biochemistry 11:3229–32.

    PubMed  Google Scholar 

  5. Lee O, Breckenridge WC (1976) Can J Biochem 54:42–49.

    PubMed  Google Scholar 

  6. Swaminathan N, Aladjem F (1976) Biochemistry 15:1516–22.

    PubMed  Google Scholar 

  7. Triplett RB, Fischer WR (1978) J Lipid Res 19:478–88.

    PubMed  Google Scholar 

  8. Attie AD, Weinstein DB, Freeze HH, Pittman RC, Steinberg D (1979) Biochem J 180:647–54.

    PubMed  Google Scholar 

  9. Shireman RB, Fischer W (1979) Biochim Biophys Acta 572:537–40.

    PubMed  Google Scholar 

  10. Vauhkonen M, Viitala J, Parkkinen J, Rauvala H (1985) Eur J Biochem 152:43–50.

    PubMed  Google Scholar 

  11. Krusius T (1976) FEBS Lett 66:86–89.

    PubMed  Google Scholar 

  12. Hatch FT, Lees RS (1968) Adv Lipid Res 6:1–68.

    PubMed  Google Scholar 

  13. Folch J, Lees M, Sloane-Stanley GA (1957) J Biol Chem 226:497–509.

    PubMed  Google Scholar 

  14. Bhatti T, Chambers RE, Clamp JR (1970) Biochim Biophys Acta 222:339–47.

    PubMed  Google Scholar 

  15. Rauvala H, Finne J, Krusius T, Kärkkäinen J, Järnefelt J (1980) Adv Carbohydr Biochem 38:389–416.

    Google Scholar 

  16. Hakomori S (1964) J Biochem (Tokyo) 55:205–8.

    Google Scholar 

  17. Finne J, Rauvala H (1977) Carbohydr Res 58:57–64.

    PubMed  Google Scholar 

  18. Viitala J, Finne J (1984) Eur J Biochem 138:393–97.

    PubMed  Google Scholar 

  19. Laemmli UK (1970) Nature 227:680–85.

    PubMed  Google Scholar 

  20. Nilsson B, Nordén NE, Svensson S (1979) J Biol Chem 254:4545–53.

    PubMed  Google Scholar 

  21. Krusius T, Finne J, Rauvala H (1976) FEBS Lett 71:117–20.

    Google Scholar 

  22. Baenziger JU, Fiete D (1979) J Biol Chem 254:2400–7.

    PubMed  Google Scholar 

  23. Carver JP, Grey AA (1981) Biochemistry 20:6607–16.

    PubMed  Google Scholar 

  24. Dorland L, Haverkamp J, Vliegenthart JFG, Spik G, Fournet B, Montreuil J (1979) Eur J Biochem 100:569–74.

    PubMed  Google Scholar 

  25. Bayard B, Kerckaert J-P (1981) Eur J Biochem 113:405–14.

    PubMed  Google Scholar 

  26. Bayard B, Montreuil J (1973) in Colloques Internationaux du Centre National de la Recherche Scientifique, No 221, 2nd Int Symp Glycoconjugates, CNRS, Paris, p 209–18.

  27. Baenziger J, Kornfeld S (1974) J Biol Chem 249:7260–69.

    PubMed  Google Scholar 

  28. Kornfeld R, Keller J, Baenziger J, Kornfeld S (1971) J Biol Chem 246:3259–68.

    PubMed  Google Scholar 

  29. Yoshina H, Furthmayr H, Kobata A (1980) J Biol Chem 255:9713–18.

    PubMed  Google Scholar 

  30. Tsuji T, Irimura T, Osawa T (1981) J Biol Chem 256:10497–502.

    PubMed  Google Scholar 

  31. Montreuil J (1982) in Comprehensive Biochemistry 19B, part II, eds. Neuberger A, van Deenen LLM, Elsevier, Amsterdam, p 1–188.

    Google Scholar 

  32. Wei C-F, Chen S-H, Yang C-Y, Marcel YL, Milne RW, Li W-H, Sparrow JT, Gotto AM Jr, Chan L (1985) Proc Natl Acad Sci USA 82:7265–69.

    PubMed  Google Scholar 

  33. Knott TJ, Rall SC Jr, Innerarity TL, Jacobson SF, Urdea MS, Levy-Wilson B, Powell LM, Pease RJ, Eddy R, Nakai H, Byers M, Priestley LM, Robertson E, Rall LB, Betsholtz C, Shows TB, Mahley RB, Scott J (1985) Science 230:37–43.

    PubMed  Google Scholar 

  34. Mahley RW, Innerarity TL, Rall SC Jr, Weisgraber KH (1984) J Lipid Res 25:1277–94.

    PubMed  Google Scholar 

  35. Neufeld EF, Ashwell G (1980) in The Biochemistry of Glycoproteins and Proteoglycans, ed. Lennarz WJ, Plenum Press, New York, p 241–66.

    Google Scholar 

  36. Finne J, Krusius T (1979) Eur J Biochem 102:583–88.

    PubMed  Google Scholar 

  37. Pollack L, Atkinson PH (1983) J Cell Biol 97:293–300.

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10 A, SF-00170, Finland

    Matti Vauhkonen

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  1. Matti Vauhkonen
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Vauhkonen, M. Complex-type carbohydrates of apolipoprotein-B of human plasma low-density lipoproteins. Glycoconjugate J 3, 35–43 (1986). https://doi.org/10.1007/BF01108610

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  • DOI: https://doi.org/10.1007/BF01108610

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