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Effects of antimycotics on the biosynthesis of cellular macromolecules inAspergillus niger protoplasts

  • Human And Animal Mycology
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Abstract

The effects of nine antimycotics on the biosynthesis of cellular macromolecules were analyzed using the regenerating system of protoplats ofAspergillus niger. The incorporation of several specific radioactive precursors into major cellular components were measured in the presence or in the absence of respective agents. Miconazole, ketoconazole, and tolnaftate inhibited the lipid synthesis. 5-Fluorocytosine strongly inhibited the DNA and protein syntheses. Griseofulvin, however, specifically inhibited the synthesis of cell wall polysaccharides, i.e. chitin and glucan. Other agents showed non-specific inhibition effects. The significance of morphological change of hypha as an indicator of antimycotic action and its feasibility as a screening tool for novel antimycotic compounds are discussed.

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Abbreviations

GRP:

germination of regenerating protoplasts

TCA:

trichloroacetic acid

References

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, Koganei, 184, Tokyo, Japan

    Kibong Oh &  Hideaki Matsuoka

  2. Department of Plant Protection, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan

    Tohru Teraoka

  3. Research and Development Division, Bio-Giken Inc., Tokyo, Japan

    Osao Sumita

  4. Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan

    Kosuke Takatori

  5. The Tokyo Kembikyoin Foundation, Tokyo, Japan

    Hiroshi Kurata

Authors
  1. Kibong Oh
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  2. Hideaki Matsuoka
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  3. Tohru Teraoka
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  4. Osao Sumita
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  5. Kosuke Takatori
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  6. Hiroshi Kurata
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Oh, K., Matsuoka, H., Teraoka, T. et al. Effects of antimycotics on the biosynthesis of cellular macromolecules inAspergillus niger protoplasts. Mycopathologia 122, 135–141 (1993). https://doi.org/10.1007/BF01103473

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  • DOI: https://doi.org/10.1007/BF01103473

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