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A study on the polysaccharide content of Ulva lactucal L.

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Abstract

A uronic acid — containing polysaccharide was isolated from the green marine algal speciesUlva lactuca by extraction with water, dilute hydrochloric acid solution, and ammonium oxalate solution. The latter agent was the best for extracting the highest amount of the polysaccharide material which contained glucuronic acid (11.4%), glucose (3.64%), arabinose (5.01%), xylose (4.55%), and rhamnose (27.33%). Partial acid hydrolysis studies and attempted fractionation on DEAE-cellulose pointed out that the polymeric carbohydrate material ofUlva lactuca may be a heteropolysaccharide. This polysaccharide appeared to contain, besides its main backbone, sequences involving glucose, arabinose and xylose.

Zusammenfassung

Aus dem Thallus der marinen GrünalgeUlva lactuca L. wurde durch Extraktion mit Wasser, verdünnter Salzsäure und Ammoniumoxalat-Lösung eine Polysaccharid-haltige Uronsäure isoliert. Als bestes Extraktionsmittel erwies sich dabei die Ammoniumoxalat-Lösung, da damit die höchsten Ausbeuten erzielt werden konnten. Das Polysaccharid-haltige Material enthielt Glucuronsäure (11,4%), Glucose (3,64%), Arabinose (5,01%), Xylose (4,55%) und Rhamnose (27,33%).

Untersuchungen über partielle Säurehydrolyse und Fraktionierung auf DEA-Cellulose ergaben, daß das polymere Kohlehydratmaterial derUlva lactuca L. ein Heteropolysaccharid sein kann. Dieses Polysaccharid enthält wahrscheinlich neben seinem Hauptbestandteil weitere Verbindungen mit Glucose, Arabinose und Xylose.

Résumé

Un uronique acide — contenant polysaccharide a été isolé d'un vert genre d'algue marinUlva lactuca par extraction avec l'eau, dilue une solution de hydrochlorique acide, et une solution d'ammonium oxalate. La dernière action a été la meilleur pour son extraction de la plus grande quantité du polysaccharide materiel qui contient glucuronique acide (11.4%), glucose (3.64%), arabinose (5.01%), xylose (4.55%), et rhamnose (27.33%). Les études concernant le partial hydrolysis acide et la fractionation essayée sur DEAE-cellulose marquent que le material du carbohydrate polymeriqued'Ulva lactuca peut être un heteropolysaccharide. Ce polysaccharide apparait qu'il contient à côté de son propre material, des successions contenant résidus de glucose, arabinose et xylose.

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References

  1. Abdel-Fattah, A. F. & Hussein, M. M. (1970). Composition of Some Brown Algae as Influenced by Seasonal Variation.Phytochem. 9:721–724.

    Google Scholar 

  2. Bitter, T. & Muir, H. M. (1962) A Modified Uronic Acid Carbazole Reaction.Analyt. Biochem. 4:330–334.

    Google Scholar 

  3. Block, R. J., Durrum, E. L. & Zweig, U. (1955). A Manual of Paper Chromatography and Paper Electrophoresis, Academic Press, New York, 127–156.

    Google Scholar 

  4. Bourne, E. J., Johnson, P. G. & Percival, E. (1970) The Water Soluble Polysaccharides ofCladophora rupestris. Part IV. Autohydrolysis, Methylation of the Partially Desulphated Material, and Correlation with the Results from SMITH Degradation.J. Chem. Soc. (C):1561–1569.

  5. Brown, A. H. (1946). Determination of Pentose in the Presence of Large Quantities of Glucose.Arch. Biochem. 11:269–278.

    Google Scholar 

  6. Dische, Z. & Shettles, L. B. (1948). A Specific Color Reaction of Methyl Pentoses and a Spectrophotometric Micromethod for their Determination.J. Biol. Chem. 175:595–603.

    Google Scholar 

  7. Dische, Z., Shettles, L. B. & Osnos, M. (1949). New Specific Color Reactions of Hexoses and Spectrophotometric micromethods for their Determination.Arch. Biochem. 22:169–184.

    Google Scholar 

  8. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances.Analyt. Chem. 28:350–356.

    Google Scholar 

  9. Fischer, F. G. & Dörfel, H. (1955a). Die Papierchromatographische Trennung and Bestimmung der Uronsäuren.Hoppe — Seyler's Z. Physiol. Chem. 301:224–234.

    Google Scholar 

  10. Fischer, F. G. & Dörfel, H. (1955b). Die Polyuronsäuren der Braunalgen.Hoppe — Seyler's Z. Physiol. Chem. 302:186–203.

    Google Scholar 

  11. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein Measurement with the Folin phenol Reagent.J. Biol. Chem. 193:265–275.

    Google Scholar 

  12. Smith, F. & Montogomery, R. (1959). The Chemistry of Plant Gums and Mucilages. Reinhold Publishing Corporation, New York, p. 455.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Microbiological Chemistry, National Research Centre, Dokki, Cairo, Egypt

    A. F. Abdel-Fattah & M. Edrees

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  1. A. F. Abdel-Fattah
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  2. M. Edrees
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Abdel-Fattah, A.F., Edrees, M. A study on the polysaccharide content of Ulva lactucal L.. Plant Food Hum Nutr 22, 15–22 (1972). https://doi.org/10.1007/BF01099733

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