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Regeneration and remyelination ofXenopus tadpole optic nerve fibres following transection or crush

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Journal of Neurocytology

Summary

Optic nerves of stage 54–56Xenopus laevis tadpoles were either transected or crushed, and subsequent Wallerian degeneration, regeneration, and remyelination were examined. After 4 days, normal myelinated fibres were no longer present in the distal stump, and only a few unmyelinated fibres remained. After 10–13 days, the distal nerve consisted mainly of a core of reactive astrocytes with enlarged processes and scattered oligodendrocytes which persisted throughout the degenerative period. Regenerating axons traversed the site of the lesion and extended into the distal stump within 13–15 days.

As regeneration progressed, astrocytic processes extended radially from the optic nerve's central cellular core and formed longitudinal compartments for regenerating axons. Between 15–19 days, a few regenerating fibres were remyelinated and by 35 days, more axons were surrounded either by thin collars of oligodendrocyte cytoplasm or by 1–3 spiral turns of myelin membrane. By 95 days, the number of myelinated fibres had increased to about 50% of those present in control nerves. Their myelin sheaths were normal in appearance and thickness relative to their respective axon diameters. The largest axons were surrounded by compact sheaths with 4–9 lamellae.

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  1. Laboratory of Neuropathology and Neuroanatomical Sciences, National Institute of Neurological Diseases and Stroke, National Institutes of Health, 20014, Bethesda, Maryland

    P. J. Reier & H. F. de Webster

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  1. P. J. Reier
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  2. H. F. de Webster
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Reier, P.J., de Webster, H.F. Regeneration and remyelination ofXenopus tadpole optic nerve fibres following transection or crush. J Neurocytol 3, 591–618 (1974). https://doi.org/10.1007/BF01097626

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  • DOI: https://doi.org/10.1007/BF01097626

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