Abstract
Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: pre-cultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre and post-freezing steps. Refining the methodology led to a consistent 50–70% plant recovery.
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Abbreviations
- DMSO :
-
Dimethylsulfoxide
- MS :
-
Murashige and Skoog medium (1962)
- LN :
-
liquid nitrogen
References
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Communicated by F. Constabel
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Escobar, R.H., Mafla, G. & Roca, W.M. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports 16, 474–478 (1997). https://doi.org/10.1007/BF01092769
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DOI: https://doi.org/10.1007/BF01092769