Abstract
The percent distributions of protein fractions namely albumin + globulin, prolamine and glutelin were studied in developing grains of NP 113 barley and its high lysine mutant Notch-2. During development the percentage of albumin + globulin fraction decreased in NP 113, while those of prolamine and glutelin remained unchanged. The increase in prolamine was substantial from 24 to 31DAA. In Notch-2 the trend followed by albumin + globulin and prolamine was like that in NP 113, while the glutelin fraction showed an increase as compared to 10 DAA. The percent of albumin + globulin was slightly higher in Notch-2 as compared to NP 113. The absolute amount (mg/grain) of all the protein fractions increased during development in both NP 113 and its mutant Notch-2. During the grain development the prolamine content was substantially lower in the mutant than in the parent NP 113. The albumin + globulin content per endosperm was in general also higher in NP 113 than Notch-2. Amino acid analysis of the protein fractions did not reveal significant changes in lysine between NP 113 and Notch-2. Thus, the improvement in lysine in the mutant is primarily due to reduced synthesis of the prolamine fraction and not due to an increase in lysine in the mutant hordein fraction. Part of the improvemenht in lysine may also be due to increase in the percentage of albumin + globulin fractions which is lysine rich.
Similar content being viewed by others
References
Cameron-Mills, V, Brandt A, Ingversen J (1980) Cereals for Food and Beverages. New York: Academic Press pp. 340–364
Brandt A (1976) Endosperm protein formation during kernel development of wild type and high lysine barley mutant. Cereal Chem 53: 890–901
Mertz ET, Bates LS, Nelson OE (1964) Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145: 279–280
Munck L, Karlsson KE, Hagberg A (1969) Proc 2nd Int Barley Genetics Symp, Washington State University Press, Barley Genetics 2: 544–558
Doll H, Koie B, Eggum BO (1974) Induced high lysine mutants in barley. Radiation Bot 14: 73–80
Bansal HC (1970) A new mutation induced in barley. Curr Sci 39: 494–501
Singh R, Axtell RD (1973) High lysine mutant gene (hl) that improves protein quality and biological value in grain sorghum. Crop Sci 13: 535–539
Balaravi SP, Bansal HC, Eggum BO, Bhaskaran S (1976) characterisation of induced high protein and high lysine mutants in barley. J Sci Food Agric 27: 545–549
Bansal HC, Srivastava KN, Eggum BO, Mehta SL (1977) Nutritional evaluation of high protein genotypes of barley. J Sci Food Agric 28: 157–160
Mehta SL, Dongre AB, Johari RP, Lodha ML, Naik MS (1979) Biochemical constraints that determine protein quality and grain yield in cereals. Seed Protein Improvement in Cereals and Grain Legumes IAEA, Vienna 1: 241–257
Sood K (1982) Biochemical and ultrastructure studies of protein and starch in high lysine barley grains. Ph.D. Thesis submitted to PG School IARI, New Delhi
Landry J, Moureax T (1970) Hetergeneite des glutelins du grasin de maes extraction selective et composition on acides amines des trois fractions isobes. Bull Soc Chem Biol 52: 1021
Sharma ND, Santha IM, Mehta SL (1985) Fatty acid and amino acid composition of groundnut mutant. Qual Plant Plant Foods Hum Nutr 35: 3–8
Pomeranz Y, Wesenberg DM, Robbins GS, Gilbertson JT (1974) changes in Amino acid composition of maturing hiproly barley. Cereal Chem 51: 635–640
Shewry PR, Prat HM, Laggat MM, Miflin BJ (1979) Protein metabolism in developing endosperms of high lysine and normal barley. Cereal Chem 56; 110–117
Bhattacharya K, Mehta SL, Banjerjee D (1986) Serological characterisation of hordein fromHordeum vulgare. Indian J Exp Biol 24: 445–448
Author information
Authors and Affiliations
Additional information
Part of the Ph.D thesis submitted by S. Joshi to P.G. School, IARI, New Delhi.
Rights and permissions
About this article
Cite this article
Joshi, S., Santha, I.M. & Mehta, S.L. Amino acid compositions of different protein fractions in developing grains of NP 113 barley and its high lysine Notch-2 mutant. Plant Food Hum Nutr 38, 277–286 (1988). https://doi.org/10.1007/BF01091725
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01091725