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Xanthophyll cycle and energy-dependent fluorescence quenching in leaves from pea plants grown under intermittent light

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Abstract

The possible role of zeaxanthin formation and antenna proteins in energy-dependent chlorophyll fluorescence quenching (qE) has been investigated. Intermittent-light-grown pea (Pisum sativum L.) plants that lack most of the chlorophyll a/b antenna proteins exhibited a significantly reduced qE upon illumination with respect to control plants. On the other hand, the violaxanthin content related to the number of reaction centers and to xanthophyll cycle activity, i.e. the conversion of violaxanthin into zeaxanthin, was found to be increased in the antenna-protein-depleted plants. Western blot analyses indicated that, with the exception of CP 26, the content of all chlorophyll a/b-binding proteins in these plants is reduced to less than 10% of control values. The results indicate that chlorophyll a/b-binding antenna proteins are involved in the energy-dependent fluorescence quenching but that only a part of qE can be attributed to quenching by chlorophyll a/b-binding proteins. It seems very unlikely that xanthophylls are exclusively responsible for the qE mechanism.

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Abbreviations

CAB:

chlorophyll a/b-binding

Chl:

chlorophyll

FV :

variable fluorescence

IML:

intermittent light

LHC:

light harvesting complex

PFD:

photon flux density

qP:

photochemical quenching of chlorophyll fluoresence

qN:

non-photochemical quenching

qE:

energy-dependent quenching

qI:

photoinhibitory quenching

qT:

quenching by state transition

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  1. Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, D-40225, Düsseldorf, Germany

    Peter Jahns & G. Heinrich Krause

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  1. Peter Jahns
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  2. G. Heinrich Krause
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Jahns, P., Krause, G.H. Xanthophyll cycle and energy-dependent fluorescence quenching in leaves from pea plants grown under intermittent light. Planta 192, 176–182 (1994). https://doi.org/10.1007/BF01089032

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  • DOI: https://doi.org/10.1007/BF01089032

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