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Control of enzymatic browning in apple slices by using ascorbic acid under different conditions

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Abstract

Control of phenol oxidase activity in apple slices by the use of ascorbic acid at different pH values, temperature and time of incubation was investigated. The enzyme was almost inactivated at 1% and 1.5% ascorbic acid. Ascorbic acid solution (1%) caused a remarkable inhibition with the increasing acidity up to pH=1. Heating treatments for apple slices dipped in 1% ascorbic acid caused a reduction of enzymatic browning, optimum temperature for inactivation of the enzyme was between 60–70°C for 15 minutes. Increasing the time of dipping apple slices in 1% ascorbic acid solutions and at different pH values reduce phenolase activity.

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References

  • Augustin MA, Ghazali HM, Hasinal Hashim (1985) Polyphenol oxidase from Guava (Psidium guajave L.). J Sci Food Agric 36: 1259

    Google Scholar 

  • Borenstein B (1965) The comparative properties of ascorbic acid and erythorbic acid. Food Technol 19: 1719

    Google Scholar 

  • Dauerfeind JC, Pinkert DM (1970) Food processing with added ascorbic acid. Adv Food Res 18: 219

    Google Scholar 

  • Eskin NAM, Henderson HM, Toznsend RJ (1971) Biochemistry of foods, Academic Press. New York, San-Fransisco, London

    Google Scholar 

  • Guandagni DO, Nimmo CC (1957) The time-temperature tolerance of frozen foods. III-Effectiveness of vacuum oxygen removal, and milk heat in controlling browning in frozen peaches. Food Technol 11: 43

    Google Scholar 

  • Joslyn MA, Ponting JD (1951) Enzyme catalyzed oxidative browning of fruit products. Advan Food Res 3: 1

    Google Scholar 

  • Ingraham LL (1956) Effect of ascorbic acid on polyphenol oxidase. J Am Chem Soc 18: 50–95

    Google Scholar 

  • Luh BS, Feinberg B and Chung JI (1975) Freezing of fruits. In ‘Commercial fruit processing’ Avipublishing Co, Inc, West Port, CT, p. 266

    Google Scholar 

  • Mapson LW, Swain I (1961) Oxidative of ascorbic acid and phenolic constituents in ‘Production and application of enzyme preparations in food manufacture’, Sci (Soc Chem Ind London). Monogr 11, p. 121

    Google Scholar 

  • Mayer AM, Hanel E (1979) Polyphenol oxidase in plant's phytochemistry, 18: 193

  • Powers MJ, Talburt WF, Jackson R, Lazar ME (1958) Dehydra canned apples. Food Technol 8: 417

    Google Scholar 

  • Rangannas M (1977) Manual of analysis of fruits and vegetable products. Tata McGraw Hill Publishing Company Limited, New Delhi

    Google Scholar 

  • Sapers MG, Dougles FW (1987) Measurement of enzymatic browning at cut surface and in juice of raw apple and pear fruits. J Food Sci 52: 1258

    Google Scholar 

  • Sapers MG, Ziolokowski AM (1987) Comparison of erythorbic and ascorbic acids as inhibitors of enzymatic browning in apples. J Food Sci 52: 1732

    Google Scholar 

  • Schultz HW (1960) Food enzymes. The Avi publishing Company Inc, West Port, CT

    Google Scholar 

  • Taeufel K and Voight J (1964) Sodium chloride as inhibitor in enzymic browning of apples. Nahrung 8: 80

    Google Scholar 

  • Vamos-Vigyaza L (1981) Polyphenol oxidase and peroxidase in fruits and vegetables. Crit Rev Food Sci Nutr 15: 49

    Google Scholar 

  • Walker LH, Power MJ, Taylor DH (1955) Factors in processing methods which affect the quality of dehydro frozen apple slices. Food Technol 9: 576

    Google Scholar 

Download references

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El-Shimi, N.M. Control of enzymatic browning in apple slices by using ascorbic acid under different conditions. Plant Food Hum Nutr 43, 71–76 (1993). https://doi.org/10.1007/BF01088098

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  • DOI: https://doi.org/10.1007/BF01088098

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