Summary
Influence of growth regulators during initiation ofMimosa tenuiflora calli tissue varied according to explant source. KN (Kinetin) affected hypocotyls, while an interaction between different combinations of 2,41) (2,4 dichlorophenoxyacetic acid) and KN induced cotyledons,stems and leaves. Calli growth after 60 days was influenced by both hormones in cotyledons, by 2,4D in hypocotils and by an interaction of the two regulators in stems and leaves. The indole alkaloid Nb-Nb dimethyltryptamine was found to be present in some cultures at a level comparable to that of the source plant.
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Lozoya X, Navarro V, Arnason JT, & Kourany E. (1989) Experimental Evaluation ofMimosa tenuiflora (Willd) Poir I. Screening of antimicrobial properties of bark extracts. Arch. Inves. Med. (Mex)20:87–90
Malmberg RL (1979) Regeneratrion of whole plants from callus cultures of diverse genetic lines ofPisum sativum L. Planta 146:243–244.
Martins-Loucao MA, & Rodríguez-Berrueco C. (1981) Establishment of proliferating callus from roots, cotyledons and hyypocotyls of carob (Creatonia siliqua L) seedlings. Z. Pflanzenphysiol. 103:297–303.
Meckes-Lozoya M, Lozoya X, & González JL (1990a) The effect produced by the alkaloidal fraction ofMimosa tenuiflora upon the peristaltic reflex in guinea pig's ileum. Arch. Inves. Med. (Mex.)21(2):171–174.
Meckes-Lozoya M, Lozoya X, & González JL (1990b) In vitro pharmacological properties of some extracts ofMimosa tenuiflora (Tepescohuite). Arch. Invest. Med. (Mex) 21(2):163–169.
Meckes-Lozoya M, Lozoya, X, Maries R, Soucy-Breau C, Varason QA & Arnason J.T. (1990c) n-n-dimethyltryptamine alkaloid inMimosa tenuiflora bark. Arch. Invest. Med. (Mex)21(2):175–177.
Meijer EGM & Broughton WJ (1981) Regeneration of whole plants from hypocotyl-root and leaf derived tissue cultures of the pasture legumeStaylosonthes guyanensis. Physiol. Plant 52:280–284.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473–497.
Villarreal ML, Nicasio P, & Alonso-Cortés D (1991) Effects ofMimosa tenuiflora bark extracts on W138 and KB human cells in culture. Arch. Invest. Med. (Mex)22(2):163–169.
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Villarreal, M.L., Rojas, G., Meckes, M. et al. Studies onMimosa tenuiflora callus culture. Interaction of kinetin and 2,4 dichlorophenoxyacetic acid in initiation and growth. Biotechnol Lett 15, 721–726 (1993). https://doi.org/10.1007/BF01080145
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DOI: https://doi.org/10.1007/BF01080145