Summary
Strains ofZymomonas mobilis containing an α-glucosidase gene cloned fromBacillus brevis strain 27-7 (NRRL B-4389) on the plasmid pNSW358 showed varying degrees of stability in batch culture under non-selective conditions. After 45 generations of growth in continuous culture, pNSW358 was stable inZ.mobilis strain ZM6100 and the specific activity of α-glucosidase in these cells was 2.7 nmol/min/mg protein. Lysed cell extracts confirmed the activity of the α-glucosidase enzyme in ZM6100(pNSW358) with 21 g/1 ethanol in 50 (82% theoretical conversion of maltose to ethanol). ZM6100(pNSW358) whole cells showed a very slow conversion rate on maltose as a sole carbon source with only 5.3 g/1 ethanol after 30 days on 100 g/l maltose medium.
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Strzelecki, A.T., Goodman, A.E., Watson, J.M. et al. Stability and expression of a cloned α-glucosidase gene inZymomonas mobilis grown in batch and continuous culture. Biotechnol Lett 15, 679–684 (1993). https://doi.org/10.1007/BF01080139
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DOI: https://doi.org/10.1007/BF01080139