Abstract
Two allergenically active components present in theAzadirachta indica whole pollen extract have been isolated by sequential ammonium sulfate precipitation (0–90%), DEAE-Sephadex A-50 ion-exchange chromatography followed by gel filtration through Sephadex G-200. The allergenicity of fractionated materials has been tested by skin prick test and ELISA inhibition which reveal that AIaI and AIaIVb are the major allergens. Immunoblot confirms the IgE-binding activity of the proteins. Although both fractions are found to be homogeneous by SDS-PAGE, isoelectric focusing produces more than one isoelectric point in AIaI (pI=3.15, 3.3 and 3.5) and AIaIVb (pI=6.0 and 6.2). Amino acid analyses of the two allergens, the effect of pH on them and cross-reactivity between them have been discussed.
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Karmakar, P.R., Chatterjee, B.P. Isolation and characterization of two IgE-reactive proteins fromAzadirachta indica pollen. Mol Cell Biochem 131, 87–96 (1994). https://doi.org/10.1007/BF01075728
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DOI: https://doi.org/10.1007/BF01075728