Abstract
After many generations of selective breeding, rats of the high-avoidance strain (SHA) average 67% avoidance responses in a two-way shuttle box, whereas those of the low-avoidance strain (SLA) average 0%. Adrenal gland weights, both absolutely and relative to body weight, are 40–50% greater in adult SLA than SHA rats of both sexes. Females of both strains have larger adrenal glands than males. Morphometry revealed that the difference in adrenal size in adults is entirely in the three cortical zones. The strain difference occurs as early as 21 days of age, whereas the sex difference appears only after puberty. A 2-min exposure to ether vapor induced an elevation in adrenal concentration of corticosterone which was significantly greater in SHA than SLA animals of both sexes at some time periods following the ether stress. Despite having smaller glands, older previously stressed SHA rats have higher basal adrenal concentrations of corticosterone than do SLA animals. The reduced steroidogenesis in the large adrenals of SLA rats suggests that there may be an enzymatic defect of genetic origin in those animals.
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Brush, F. R., Froehlich, J. C., and Sakellari, P. C. (1979). Genetic selection for avoidance behavior in the rat.Behav. Genet. 9:309–316.
Brush, F. R., Froehlich, J. C., and Baron, S. (1981). Hormonal mediation of genetic differences in avoidance behavior. In Stark, E., Makara, G. B., Acs, Zs., and Endroczi, E. (eds.),Advances in Physiological Sciences, Vol. 12., Endocrinology, Neuroendocrinology, Neuropeptides-1; Akdemiai Kiado, Budapest, pp. 269–272.
Brush, F. R., Baron, S., Froehlich, J. C., Ison, J. R., Pellegrino, L. J., Phillips, D. S., Sakellaris, P. C., and Williams, V. N. (1985). Genetic differences in avoidance learning byRattus norvegicus: Escape/avoidance responding, sensitivity to electric shock, discrimination learning and open-field behavior.J. Comp. Psychol. 99:60–73.
Brush, F. R., Del Paine, S. N., Pellegrino, L. J., Rykaszewski, I. M., Dess, N. K., and Collins, P. Y. (1988). CER suppression, passive avoidance learning, and stressinduced suppression of drinking in the Syracuse high-and low-avoidance strains of rats (Rattus norvegicus)J. Comp. Psychol. 102:337–349.
Farese, R. V., and Reddy, W. Y. (1963). Observations on the interrelations between adrenal protein, RNA and DNA during prolonged ACTH administration.Biochim. Biophys. Acta 76:145–148.
Feuer, G. (1963). Adrenal cortical activity and stress response in rats selectively bred for differences in behaviour.J. Physiol. 169:43P-44P.
Fiala, S., Sproul, E. E., and Fiala, A. (1956). The action of corticotropin (ACTH) on nucleic acids and subcellular elements of the adrenal cortex.J. Biophys. Biochem. Cytol. 2:115–126.
Froehlich, J. C. (1978).The Effects of Changes in Feeding Regimen and Hormonal Pretreatment on the Half-Life of Corticosterone in the Freely-Moving Rat, Unpublished doctoral dissertation, Syracuse University, Syracuse, N. Y.
Gentsch, C., Lichsteiner, M., and Feer, H. (1981). Locomotor activity, defecation score and corticosterone levels during openfield exposure: A comparison among individually and group-housed rats, and genetically selected rat lines.Physiol. Behav. 27:183–186.
Gentsch, C., Lichsteiner, M., Driscoll, P., and Feer, H. (1982). Differential hormonal and physiological responses to stress in Roman high-and low-avoidance rats.Physiol. Behav. 28:259–263.
Glick, D., Von Redlich, D., and Levine, S. (1964). Fluorometric determination of corticosterone and cortisol in 0.02–0.05 milliliters of plasma or submilligram samples of adrenal tissue.Endocrinology 74:653–655.
Imrie, R. X., Ramaih, T. R., Antonini, F., and Hutchison W. C. (1965). The effect of adrenocorticotrophin on the nucleic acid metabolism of the rat adrenal gland.J. Endocrinol. 32:303–312.
Jones, M. T., Tiptaft, E. M., Brush, F. R., Fergusson, D. A. N., and Neame, R. L. B. (1974). Evidence for dual corticosteroid-receptor mechanisms in the feedback control of adrenocorticotrophin secretion.J. Endocrinol. 60:223–233.
Levine, S., and Mullins, R. F., Jr. (1968). Hormones in infancy. In Newtons, G., and Levine, S. (eds.),Early Experience and Behavior, C. C Thomas, Springfield, Ill., pp. 168–197.
Rogers, P. V., and Richter, C. P. (1948). Anatomical comparisons between the adrenal glands of wild Norway, wild Alexandrine and domestic Norway rats,Endocrinology 42:46–55.
Shapiro, S. (1968). Maturation of the neuroendocrines response to stress in the rat. In Newton, G., and Levine, S. (eds.),Early Experience and Behavior, C. C. Thomas, Springfield, Ill., pp. 198–257.
Walker, C.-D., Rivest, R. W., Meaney, M. J., and Aubert, M. L. (1989) Differential activation of the pituitary-adrenocortical axis after stress in the rat: Use of two genetically selected lines (Roman Low-and High-Avoidance rats) as a model.J. Endocrinol. 123:477–485.
Yeakel, E. H., and Rhoades, R. P. (1941). A comparison of the body and endocrine gland (adrenal, thyroid and pituitary) weights of emotional and non-emotional rats.Endocrinology 28:337–340.
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This research was supported in part by David Ross Fellowships from the Purdue University Research Foundation to M. D. Isaacson and I. M. Rykaszewski and by Research Grant MH39230 to F. R. Brush from the National Institute of Mental Health.
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Brush, F.R., Isaacson, M.D., Pellegrino, L.J. et al. Characteristics of the pituitary-adrenal system in the syracuse high-and low-avoidance strains of rats (Rattus norvegicus). Behav Genet 21, 35–48 (1991). https://doi.org/10.1007/BF01067665
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DOI: https://doi.org/10.1007/BF01067665