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Combined antitumor effects of TNF and G-CSF on a human medulloblastoma xenograft line

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The antitumor effects of TNF and G-CSF on a xenograft line of human medulloblastoma were examined. (Method): 1) A human medulloblastoma xenograft line was transplanted into nude mice. Tumor bearing nude mice were divided into the following eight groups: untreated controls (C); those receiving a subcutaneous injection of G-CSF for one week (G1); for four weeks (G2); those receiving an intratumoral injection of TNF for four weeks (Tit); an intravenous injection of TNF (Tiv); those receiving a combination of G1 and Tit (G1 + Tit); a combination of G2 and Tit (G2 + Tit); and a combination of G2 and Tiv (G2 + Tiv). The relative tumor weight in each group was calculated and any antitumor effects were examined by calculating a tumor growth inhibition ratio. 2) Tumor bearing nude mice were divided into the following two groups: those receiving a subcutaneous injection of G-CSF and an intravenous injection of TNF (G + T); and only an intravenous injection of TNF (T). We evaluated the pathological findings from the tumors at 0 h, 0.5 h, 1 h, 3 h, 6 h, 12 h, 24 h and 48 h after the TNF injection. Routine H.E. staining and immunostaining using antigranulocyte and antimacrophage antibodies were performed. (Results): 1) The tumor growth inhibition ratio was 0.112, 0.190, 0.287, 0.451, 0.347, 0.635, and 0.622 at G1, G2, Tit, Tiv, G1 + Tit, G2 + Tit, G2 + Tiv group. A combined antitumor effect was clearly seen in the G2 + Tit and the G2 + Tiv groups. 2) The tumor was fragmented by the infiltration of many inflammatory cells 24 hours after TNF injection. Many more macrophages were observed in the tumors of G + T mice than in the T mice. Granulocytes were observed only in the tumors of the G + T mice.

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Authors and Affiliations

  1. Department of Neurosurgery, Hiroshima University School of Medicine, Hiroshima, Japan

    Hitoshi Maeda, Tohru Uozumi, Kaoru Kurisu, Takashi Matsuoka, Keiichi Kawamoto, Katsuzo Kiya, Hidenori Ogasawara, Kazuhiko Sugiyama, Takashi Mikami, Syuji Monden, Kunyu Harada & Yasuhiro Matsuda

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  1. Hitoshi Maeda
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  2. Tohru Uozumi
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  3. Kaoru Kurisu
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  4. Takashi Matsuoka
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  5. Keiichi Kawamoto
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  7. Hidenori Ogasawara
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  12. Yasuhiro Matsuda
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Maeda, H., Uozumi, T., Kurisu, K. et al. Combined antitumor effects of TNF and G-CSF on a human medulloblastoma xenograft line. J Neuro-Oncol 21, 203–213 (1994). https://doi.org/10.1007/BF01063769

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