Abstract
The present study examines the structure of the lysosomal system of mature oocytes in mussels,Mytilus galloprovincialis, after a 21 day exposure to the water accommodated fraction (WAF) of two crude oils (types Ural and Maya) and of a commercial lubricant oil. The automated image analysis indicates that lysosomes, showing cytochemically demonstrable β-glucuronidase activity, are smaller and much more numerous in oocytes of mussels treated with a 40% dose of Ural- and Lubricant-WAF when compared to controls. It is suggested that the structure of the lysosomal system of oocytes is different from that of somatic cells (i.e., digestive cells) and that budding or “fission” into smaller bodies occurs in oocyte lysosomes under certain petroleum hydrocarbon-exposure conditions. These changes in the lysosomal compartment appear to be associated to the process of gamete release or spawning.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson JW, Neff JM, Cox BA, Tatem HE, Hightower GM (1974) Characteristics of dispersions and water-soluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. Mar Biol 27:75–88
Bayne BL, Holland DL, Moore MN, Lowe DM, Widdows J (1978) Further studies on the effect of stress in the adult on the eggs ofMytilus edulis. J mar Biol Ass UK 58:825–843
Berthou F, Balouet G, Bodennec G, Marchand M (1987) The occurrence of hydrocarbons and histopathological abnormalities in oysters for seven years following the wreck of the Amoco Cadiz in Brittany (France). Mar Environ Res 23:103–133
Besten PJ den, Herwig HJ, Zandee DI, Voogt PA (1989) Effects of cadmium and PCBs on reproduction of the sea starAsterias rubens: aberrations in the early development. Proc 1st Eur Conf Ecotox, pp 167–168
Bolognani AM, Albanese Carmignani MP, Zaccone G, Minniti F (1979) Cytochemical detection of some enzymes of the carbohydrate metabolism in the yolk of molluscan oocytes. Cell molec Biol 24:265–266
Boylan DB, Tripp BW (1971) Determination of hydrocarbons in seawater extracts of crude oil and crude oil fractions. Nature 230:44–47
Cajaraville MP, Díez G, Marigómez JA, Angulo E (1990b) Responses of basophilic cells of the digestive gland of mussels to petroleum hydrocarbon exposure. Dis Aquat Org 9:221–228
Cajaraville MP, Marigómez JA, Angulo E (1989) A stereoiogical survey of lysosomal structure alterations inLittorina littorea exposed to 1-naphthol. Comp Biochem Physiol 93C:231–237
—, —, — (1990a) Short-term toxic effects of 1naphthol on the digestive gland-gonad complex of the marine prosobranchLittorina littorea (L): a light microscopic study. Arch Environ Contam Toxicol 19:17–24
—, —, — (1990c) Ultrastructural study of the shortterm toxic effects of naphthalene on the kidney of the marine prosobranchLittorina littorea. J Invertebr Pathol 55:215–224
Carles D, Henry M, Siron R, Giusti G (1986) Induction of ultrastructural alterations in the digestive gland ofMytilus galloprovincialis (Lamarck) by crude oil. In: Giam CS, Dou HJ-M (eds) Strategies and advanced techniques for marine pollution studies: Mediterranean Sea. NATO ASI Series, Vol G9, Springer-Verlag, Berlin/Heidelberg, pp 461–462
Dorange G, Le Pennec M (1989) Ultrastructural study of oogenesis and oocytic degeneration inPecten maximus from the Bay of St. Brieuc. Mar Biol 103:339–348
Heuser J (1989) Changes in lysosome shape and distribution correlated with changes in cytoplasmic pH. J Cell Biol 108:855–864
Holtzman E (1989) Lysosomes. Plenum Press, NY, 439 pp
Hummel H, UitOudeGroeneveld JP, Nieuwenhuize J, Liere JM van, Bogaards RH, Wolf L de (1989) Relationship between PCB concentrations and reproduction in musselsMytilus edulis. Mar Environ Res 28:489–493
Jong-Brink M de, Geraerts WPM (1982) Oogenesis in gastropods. Malacologia 22:145–149
Jong-Brink M de, Wit A de, Kraal G, Boer HH (1976) A light and electron microscope study on oogenesis in the freshwater pulmonate snailBiomphalaria glabrata. Cell Tiss Res 171:195–219
Kerr JFR (1973) Some lysosome functions in liver cells reacting to sublethal injury. In: Dingle JT (ed) Lysosomes in biology and pathology, Vol 3, North-Holland/American Elsevier, Amsterdam, pp 365–394
Lowe DM, Moore MN, Clarke KR (1981) Effects of oil on digestive cells in mussels: quantitative alterations in cellular and lysosomal structure. Aquat Toxicol 1:213–226
Lowe DM, Pipe RK (1986) Hydrocarbon exposure in mussels: a quantitative study of the responses in the reproductive and nutrient storage cell systems. Aquat Toxicol 8:265–272
—, — (1987) Mortality and quantitative aspects of storage cell utilization in mussels,Mytilus edulis, following exposure to diesel oil hydrocarbons. Mar Environ Res 22:243–251
Marigómez JA, Vega MM, Cajaraville MP, Angulo E (1989) Quantitative responses of the digestive lysosomal system of winkles to sublethal concentrations of cadmium. Cell molec Biol 35:555–562
Moore MN (1976) Cytochemical demonstration of latency of lysosomal hydrolases in digestive cells of the common mussel,Mytilus edulis, and changes induced by thermal stress. Cell Tiss Res 175:279–287
— (1985) Cellular responses to pollutants. Mar Pollut Bull 16:134–139
— (1988) Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Mar Ecol Prog Ser 46:81–89
Moore MN, Pipe RK, Farrar SV, Thomson S, Donkin P (1987) Lysosomal and microsomal responses to oil derived hydrocarbons inLittorina littorea. In: Capuzzo JR, Kester DR (eds) Oceanic processes in marine pollution. RE Krieger, Malabar, FL, pp 89–96
Peek K, Gabbott PA (1990) Seasonal cycle of lysosomal enzyme activities in the mantle tissue and isolated cells from the musselMytilus edulis. Mar Biol 104:403–412
Pipe RK (1985) Seasonal cycles in and effects of starvation on egg development inMytilus edulis. Mar Ecol Prog Ser 24:121–128
— (1987) Oogenesis in the marine musselMytilus edulis: an ultrastructural study. Mar Biol 95:405–414
Pipe RK, Moore MN (1985) The ultrastructural localization of lysosomal acid hydrolases in developing oocytes of the common marine musselMytilus edulis. Histochem J 17:939–949
Renzoni A (1975) Toxicity of three oils to bivalve gametes and larvae. Mar Pollut Bull 6:125–128
Robinson AG, Dillaman RM (1985) The effects of naphthalene on the ultrastructure of the hepatopancreas of the fiddler crab,Uca minax. J Invertebr Pathol 45:311–323
Sokal RR, Rohlf FJ (1979) Biometria. H Blume Ediciones, Madrid, 832 pp
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cajaraville, M.P., Marigómez, J.A. & Angulo, E. Automated measurement of lysosomal structure alterations in oocytes of mussels exposed to petroleum hydrocarbons. Arch. Environ. Contam. Toxicol. 21, 395–400 (1991). https://doi.org/10.1007/BF01060362
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01060362