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The RG2 rat glioma model

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Summary

The ethylnitrosourea-induced cell line RG2 grows very well in infinite cell culturein vitro, and provides a simple, reproducible glioma model when inoculated into the brains of syngeneic Fischer 344 rats. We have used this tumor model in a series of therapy studies. We here report our experiences of the untreated (= tumor bearing control) animals, e.g. in terms of the techniques employed and also the growth, histology and effects upon the blood-brain barrier of the tumors. Weight loss as a measure of systemic effects during tumor development is also described. The RG2 model has considerable potential as a suitable tool for experimental neuro-oncology.

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References

  1. Bigner DD, Swenberg JA; JÄnisch and Schreiber's Experimental Tumors of the Central Nervous System. The Upjohn Company, Michigan, 1977

    Google Scholar 

  2. Wechsler W, Kleihues P, Matsumoto S, Zülch KJ, Ivankovic S, Preussman R, Druckrey H: Pathology of experimental neurogenic tumors chemically induced during prenatal and postnatal life. Ann NY Acad Sci 159: 360–408, 1969

    Google Scholar 

  3. Groothuis DR, Fischer JM, Lapin G, Bigner DD, Vick NA: Permeability of different experimental brain tumor models to horseradish peroxidase. J Neuropathol Exp Neurol 41: 164–185, 1982

    PubMed  Google Scholar 

  4. Groothuis DR, Fischer JM, Pasternak JF, Blasberg RG, Bigner DD: Regional measurements of blood-to-tissue transport in transplanted RG2 rat gliomas. Cancer Res 43: 3368–3373, 1983

    PubMed  Google Scholar 

  5. Groothuis DR, Pasternak J, Fischer J, Blasberg RG, Bigner DD, Vick NA: Regional measurements of blood flow in experimental RG2 rat gliomas. Cancer Res 43: 3362–3367, 1983

    PubMed  Google Scholar 

  6. Hossman KA, Mies G, Paschen W, Szabo L, Dolan E, Wechsler W: Regional metabolism of experimental brain tumors. Acta Neuropathol (Berl) 69: 139–147, 1986

    Google Scholar 

  7. Krajewski S, Kiwit JC, Wechsler W: RG2 glioma growth in rat cerebellum after subdural implantation. J Neurosurg 65 (2) 222–229, 1986

    PubMed  Google Scholar 

  8. Wechsler W, Teske U, Reifenberger G, Deckert M, Seitz RJ, Mies G, Paschen W, Hossman K-A: Neuropathology and regional imaging of microcirculation, tissue pH, metabolites and necroses in cerebral RG2 and F98 anaplastic rat glioma transplantation tumors. In: Chatel M, Darcel F, Pecker J (eds) Brain Oncology, Martinus Nijhoff Publ Dordrecht, 1987, 187–199

  9. Bilzer T, Reifenberger G, Wechsler W: Chemical induction of brain tumors in rats by nitrosoureas: Molecular biology and neuropathology. Neurotoxicol Teratol 11(6): 551–556, 1989

    PubMed  Google Scholar 

  10. Schuepbach D, Gavin PR, Salford LG, Ceberg C, Fankhauser H: RG2 rat glioma model: influence of the blood-brainbarrier on biodistribution of boronsulfhydryl. In: Barth RF, Soloway AH (eds) Advances in Neutron Capture Therapy, Plenum Press, New York, 1993, pp 485–491

    Google Scholar 

  11. Andersson-Engels S, Brun A, Kjellén E, Salford LG, Strömblad LG, Svanberg K, Svanberg S: Identification of brain tumors in rats using laser-induced fluorescence and haematoporphyrin derivative. Lasers in Med Science 4: 241–249, 1989

    Google Scholar 

  12. Ceberg CP, Persson A, Brun A, Jergil B, Persson RBR, Salford LG: Macroscopic and microscopic biodistribution of BSH in a rat glioma model. In: Gable D, Moss R (eds) Towards Clinical Trials of Glioma with BNCT. Plenum Press, New York, 1992, pp 175–179

    Google Scholar 

  13. Ceberg C, Salford LG, Brun A, Hemler R, Persson BRR: Neutron capture imaging of10B in tissue specimens. J Radiother Oncol 26: 139–146, 1993

    Google Scholar 

  14. Aas AT, Brun A, Pero RW, Salford LG: Chlorpromazine in combination with nitrosourea inhibits experimental glioma growth. Br J Neurosurg 5: 187–192, 1994

    Google Scholar 

  15. Aas AT, Salford LG, Lönn S, Lönn U: Reduced formation of high molecular weight DNA in murine gliomas treated with nitrosourea and chlorpromazine. Anticancer Res 14: 1133–1136, 1994

    PubMed  Google Scholar 

  16. Aas AT, Tönnessen TI, Brun A, Salford LG: Growth inhibition of rat glioma cellsin vitro andin vivo by aspirin. This article will be published in Journal of Neuro-Oncology 24: 171–180, 1995

    Google Scholar 

  17. Hemler R, Ceberg C, Brun A, Gable D, Persson BRR, Salford LG: A quantitative study on biokinetics and biodistribution of BSH in a rat glioma model. In: Allen BJ, Moore DE (eds) Neutron Capture Therapy for Cancer. Plenum Press, New York 1992, pp 469–472

    Google Scholar 

  18. Salford LG, Pero RW, Aas AT, Brun A: Metoclopramide as a sensitizer of 1,3-bis(2-Chloroethyl)-1-nitrosourea treatment of brain tumors in the rat. Anti-Cancer drugs 3: 267–272, 1992

    PubMed  Google Scholar 

  19. Salford LG, Brun A, Eberhardt JL, Persson BRR: Experimental studies of brain tumour development during exposure to continuous and pulsed 915 MHz-radiofrequency radiation. Bioelectrochemistry and Bioenergetics 30: 313–318, 1993

    Google Scholar 

  20. Salford LG, Brun A, Eberhardt JL, Persson BRR: Experimental studies of brain tumor development during exposure to continuous and pulsed 915 MHz radiofrequency radiation. In: Martin Blank (ed) Electricity and Magnetism in Biology and Medicine, San Francisco Press Inc. 1993, pp 379–381

  21. Salford LG, Brun A, Ceberg C, Kongstad P, Mir LM, Persson BRR: Electroporationin vivo — a new tool in the treatment of malignant brain tumors. Uppsala Journal of Medical Science. Suppl 52, p 66, 1993

    Google Scholar 

  22. Salford LG, Persson BRR, Brun A, Ceberg C, Kongstad P, Mir L: A new brain tumour therapy combining bleomycin within vivo electropermeabilization. Biochem Biophys Res Commun Vol 194, 2: 938–943, 1993

    PubMed  Google Scholar 

  23. Weber DA, Ivanovic M, Franceschi D, Strand S-E, Erlandsson K, Atkins HL, Coderre JA, Susskind H, Button T, Ljunggren K: Pinhole SPECT: An approach toin vivo high resolution SPECT imaging in small laboratory animals. J Nucl Med 35: 342–348, 1994

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Experimental Neurooncology, Department of Neurosurgery, University Hospital, Lund, Sweden

    Alf Tore Aas, Catharina Blennow, Susanne Strömblad & Leif G. Salford

  2. Division of Neuropathology, Department of Pathology, University Hospital, Lund, Sweden

    Arne Brun

Authors
  1. Alf Tore Aas
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  2. Arne Brun
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  3. Catharina Blennow
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  4. Susanne Strömblad
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  5. Leif G. Salford
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Aas, A.T., Brun, A., Blennow, C. et al. The RG2 rat glioma model. J Neuro-Oncol 23, 175–183 (1995). https://doi.org/10.1007/BF01059948

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