Abstract
The effects of acute Stressors on the concentration of free amino acids (FAA) in the freshwater amphipodGammarus pseudolimnaeus was investigated. Forty-eight hours of exposure to acutely toxic concentrations of pentachlorophenol resulted in a significant decrease in the total FAA pool at the greater concentrations and a significant change in the FAA profile at the lesser concentration. Hyperosmotic conditions did not alter the FAA concentrations while hypoosmotic conditions caused a significant decrease in the total FAA pool. Pentachlorophenol exposure did not impair the ability of amphipods to resist hypoosmotic conditions; however, osmotic stress did influence the organisms ability to recover from toxicant exposure, as measured by changes in the concentration of FAA. The observed alterations in the concentration of total FAA in stressed amphipods is probably related to a disruption in the osmoregulatory ability of the organism. Changes in the FAA pool has potential as ininsitu biochemical indicator of toxicant-induced stress in freshwater invertebrates.
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Augenfeld JM, Anderson JW, Woodruff DL, Webster JL (1980) Effects of Prudhoe Bay crude oil—contaminated sediments onProtothaca staminea: Hydrocarbon content, condition index, free amino acid level. Mar Environ Res 4:135–143
Bayne BL (1973) Physiological changes inMytilus edulis induced by temperature and nutritive stress. J Mar Biol Assoc UK 53:39–58
Bayne BL, Brown DA, Burns K, Dixon DR, Ivanovici A, Livingstone DR, Lowe DM, Moore MN, Stebbing ARD, Widdows J (1985) The effects of stress and pollution on marine animals. Praeger Publishers, New York, 384 pp
Bhagyalakshmi A, Sreenivasula Reddy P, Ramamurthi R (1983) Muscle nitrogen metabolism of freshwater crab,Oziotelphusa senex senex, Fabricuis, during acute and chronic Sumithion® intoxication. Toxicol Lett 17:89–93
Bursell E (1966) Aspects of the flight metabolism of tsetse flies (Glossina) Comp Biochem Physiol 19:809
Carr RS, Thomas P, Neff JM (1982) A simple spectrophotometric technique for the determination of pentachlorophenol in water. Bull Environ Contam Toxicol 28:477–479
Duchateau-Bosson GH, Florkin M (1961) Change in intracellular concentration of free amino acids as a factor of euryhalinity in the crayfishAstacus astacus. Comp Biochem Physiol 3:245–249
Gardner WS, Miller WH, Imlay MJ (1981) Free amino acids in mantle tissues of the bivalveAmblema plicata: Possible relation to environmental stress. Bull Environ Contam Toxicol 26:157–162
Gilles R (1970) Intermediary metabolism and energy production in some invertebrates. Arch Inter de Physiol de Biochem 78:313–326
— (1975) Mechanisms of iono- and osmoregulation. In: Kinne O (ed) Marine Ecology, Vol 2(1), Wiley Intrascience, New York, pp 259–347
— (1979) Intracellular organic osmotic effectors. In: Gilles R (ed) Mechanism of Osmoregulation in Animals, Maintenance of Cell Volume. John Wiley and Sons, New York, pp 111–154
Gould E, Collier RS, Karolus JJ, Givens S (1976) Heart transaminase in the rock crab,Cancer irroratus, exposed to cadmium salts. Bull Environ Contam Toxicol 15:635–643
Graney RL (1986) Toxicant-induced alterations in the free amino acid pool of freshwater invertebrates. PhD Dissertation, Michigan State University, E. Lansing, MI
Graney RL, Gieshy JP (1986) Seasonal variation in the free amino acid pool of the freshwater amphipodGammarus pseudolimnaeus Bousfield. Comp Biochem Phys (In press)
Guidotti GG, Borghetti AF, Gazzola GC (1978) The regulation of amino acid transport in animal cells. Biochem Biophys Acta 515:329–366
Hanson JA, Dietz TH (1976) The role of free amino acids in cellular osmoregulation in the freshwater bivalve (Ligumia subrostrata) (Say). Can J Zool 54:1927–1931
Holmberg B, Jensen S, Larsson A, Lewander K, Olsson M (1972) Metabolic effects of technical pentachlorophenol (PCP) on the eelAnguilla anguilla L. Comp Biochem Physiol 43B:171–183
Jacob R, Barrett E (1982) Chromatographic analysis of glutamine in plasma. J Chromatogr 229:188–192
Jeffries HP (1972) A stress syndrome in the hard clam,Mercenaria mercenaria, J Invert Pathol 20:242–251
Kasschau MR, Skaggs MM, Chen ECM (1980) Accumulation of glutamate in sea anemones exposed to heavy metals and organic amines. Bull Environ Contam Toxicol 25:873–878
Morrison DF (1967) Multivariate Statistical Methods. McGraw-Hill, New York, 338 pp
Munro HN (1970) Free amino acid pools and their role in regulation. In: Munro HN (ed) Mammalian Protein Metabolism, Academic Press, New York, pp 299–386
Peer MM, Nirmala J, Kutty MN (1983) Effects of pentachlorophenol (NaPCP) on survival, activity and metabolism inRhionmugil corsula (Hamilton),Cyprinus carpio (Linnaeus) andTilapia mossambica (Peters). Hydrobiologia 107:19–24.
Powell EN, Kasschau M, Chen E, Koenig M, Pecon J (1982) Changes in the free amino acid pool during environmental stress in the gill tissue of the oyster,Crassostrea virginica. Comp Biochem Physiol 71A:591–598
Riley RT, Mix MC (1981) The effects of naphthalene on glucose metabolism in the European flat oysterOstrea edulis. Comp Biochem Physiol 70C: 13–20
Roesijadi G, Anderson JW (1979) Condition index and free amino acid content ofMacoma inquinata exposed to oil contaminated marine sediments. In: Vernberg WE, Calabrese A, Thurberg FP and Vernberg FJ (eds) Marine Pollution: Functional Responses. Academic Press, New York, pp 69–83
Roesijadi G, Anderson JW, Giam CS (1976) Osmoregulation of the grass shrimpPalaememetes pugio exposed to polychlorinated biphenyls (PCBs) II. Effect on free amino acids of muscle tissue. Mar Biol 38:357–363
Sansone G, Biondi A, Noviello L (1978) Free amino acids in fluids and tissues ofMytilus galloprovincialis in relation to the environment. Their behavior as an index of normality. Comp Biochem Physiol 61A:133–139
SAS Institute Inc (1982) SAS Users Guide: Statistics, 1982 edition. SAS Institute Inc, Raleigh, NC, 584 pp
Stebbing ARD (1981) Stress, health and homeostasis. Mar Pollut Bull 12:326–329
Stryer L (1981) Biochemistry. WH Freeman, San Francisco, CA, 949 pp
Weinbach EC (1956) The influence of pentachlorophenol on oxidative and glycolytic phosphorylation in snail tissue. Arch Biochem Biophys 64:129–143.
Weinbach EC, Nolan MO (1956) The effect of pentachlorophenol on the metabolism of the snailAustiaborbis glabratus. Exp Parasitol 5:276–284
Widdows J, Bayne BL, Donkin P, Livingstone DR, Lowe DM, Moore MN, Salkeld PN (1981) Measurement of the responses of mussels to environmental stress and pollution in Sullom Voe: A base-line study. Proc Royal Soc Edinburgh 80B:323–338
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Graney, R.L., Giesy, J.P. The effect of short-term exposure to pentachlorophenol and osmotic stress on the free amino acid pool of the freshwater amphipodGammarus pseudolimnaeus Bousfield. Arch. Environ. Contam. Toxicol. 16, 167–176 (1987). https://doi.org/10.1007/BF01055798
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DOI: https://doi.org/10.1007/BF01055798