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Monoclonal antibodies specific for type 3 and type 4 chain-based blood group determinants: Relationship to the A1 and A2 subgroups

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Abstract

Two monoclonal antibodies, specific for A type 3 and A type 4 blood group determinants, are described. These antibodies recognized A1 but not A2 erythrocytes. A third monoclonal antibody showing specificity for A type 3 and A type 4, and also for H type 3 and H type 4, did not discriminate between A1 and A2 erythrocytes. On red cells these three antibodies recognized glycosphingolipids and binding to glycoproteins could not be demonstrated. On paraffin-embedded tissue sections the three antibodies labelled a supranuclear area, characteristic of the Golgi apparatus, of all cells producing A antigens. This labelling occurred irrespective of the A1, A2 status.

The results suggest that glycolipids of erythrocytes and possibly of other cell types bear the A type 3/4 determinant specific for the A1 subgroup and that A type 3/4 determinants of glycoproteins might be present in both A1 and A2 subgroups on short oligosaccharide chains which are only detectable at the level of the Golgi apparatus.

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Authors and Affiliations

  1. Institut d'Immunobiologie, Hôpital Broussais, 75674, Paris Cedex 14, France

    Jacques Le Pendu, Francine Lambert & Rafael Oriol

  2. Department of Medical Biochemistry, University of Göteborg, Sweden

    Bo Samuelsson

  3. Department of Surgery I, Sahlgrens Hospital, University of Göteborg, Sweden

    Michael E Breimer

  4. Abteilung für Klinische Immunopathologie, Universitäts-Kinder Klinik, Hamburg, W. Germany

    R Christiane Seitz & Alois Poschmann

  5. Knickerbocker S.A.E., Barcelona, Spain

    Maria Pilar Urdaniz, Nuria Suesa & Josep Vinas

  6. Chembiomed Ltd, University of Alberta, Edmonton, Canada

    Murray Ratcliffe

  7. Centre de Transfusion Sanguine, Hôpital Broussais, Paris, France

    Anne Francois

Authors
  1. Jacques Le Pendu
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  2. Francine Lambert
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  3. Bo Samuelsson
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  4. Michael E Breimer
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  5. R Christiane Seitz
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  6. Maria Pilar Urdaniz
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  7. Nuria Suesa
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  8. Murray Ratcliffe
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  9. Anne Francois
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  10. Alois Poschmann
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  11. Josep Vinas
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  12. Rafael Oriol
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Le Pendu, J., Lambert, F., Samuelsson, B. et al. Monoclonal antibodies specific for type 3 and type 4 chain-based blood group determinants: Relationship to the A1 and A2 subgroups. Glycoconjugate J 3, 255–271 (1986). https://doi.org/10.1007/BF01051776

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  • DOI: https://doi.org/10.1007/BF01051776

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