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Determination of the structure of sulfated tetra- and pentasaccharides obtained by alkaline borohydride degradation of hen ovomucin. A fast atom bombardment-mass spectrometric and1H-NMR spectroscopic study

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Abstract

Alkaline borohydride reductive cleavage of hen ovomucin resulted in the release of a series of neutral and acidic oligosaccharide-alditols.1H-NMR spectroscopy in combination with fast ion bombardment-mass spectrometry in negative ion mode were used for investigation of the structures of three oligosaccharide-alditols. The following structures were established:

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Abbreviations

NeuAc:

N-acetyl-d-neuraminic acid

Gal:

d-galactose

GlcNAc:

N-acetyl-d-glucosamine

Gal-NAc-ol:

N-acetyl-d-galactosaminitol

NMR:

nuclear magnetic resonance

FAB-MS:

fast atom bombardmentmass spectrometry

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique (Laboratoire National et Unité Associée au C.N.R.S. no. 217) de l'Université des Sciences et Techniques de Lille Flandres-Artois, F-59655, Villeneuve d'Ascq, France

    Gérard Strecker, Jean-Michel Wieruszeski, Claude Martel & Jean Montreuil

Authors
  1. Gérard Strecker
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  2. Jean-Michel Wieruszeski
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  3. Claude Martel
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  4. Jean Montreuil
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Strecker, G., Wieruszeski, JM., Martel, C. et al. Determination of the structure of sulfated tetra- and pentasaccharides obtained by alkaline borohydride degradation of hen ovomucin. A fast atom bombardment-mass spectrometric and1H-NMR spectroscopic study. Glycoconjugate J 4, 329–337 (1987). https://doi.org/10.1007/BF01048366

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  • DOI: https://doi.org/10.1007/BF01048366

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