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The mapping of natural boron in histological sections of mouse tissues by the use of neutron-capture radiography

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Summary

Neutron-capture radiography has been applied to the mapping of natural boron in mouse histological sections. The method is based on the fact that the stable isotope boron-10 has an extremely large cross-section for the thermal-neutron reaction10B(n, α)7Li. The local tissue boron concentrations were evaluated from the corresponding track densities of the nuclear reaction using an equation derived from a theoretical model of the system. The adjustable parameters of this equation were determined in two separate calibration experiments using standardized samples prepared by the addition of known amounts of boron. Interference from other nuclides engaged in nuclear reactions with thermal neutrons was also estimated. In the present experimental conditions the natural boron lower detection limit was 0.03 p.p.m. (fresh weight), and the spatial resolution was of the order of a few micrometers. Boron concentrations in mouse serum and urine were close to 0.22 and 0.57 μg ml−1, respectively. In the solid mouse tissues-liver, heart, brain, muscle and spleen-the concentration was usually low, ranging from 0.12 to 0.16 p.p.m. (fresh weight). They were significantly higher in the kidney, especially in the papilla (6.2 p.p.m. fresh weight). Apart from the kidney papilla, where boron was particularly concentrated in wall tubules, the boron distribution in most tissues appeared to be practically homogeneous. Natural boron, and even more so its enriched stable isotope10B, appear as good candidates for molecular labelling using non-radioactive tracers.

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References

  • Abe, M., Amano, K., Kitamura, K., Tateishi, J. &Hatanaka, H. (1986) Boron distribution analysis by alpha-autoradiography.J. Nucl. Med. 27, 677–84.

    PubMed  Google Scholar 

  • Ausserer, W. A., Ling, Y. C., Chandra, S. &Morrison, G. H. (1989) Quantitative imaging of boron, calcium, magnesium, potassium and sodium distributions in cultured cells with ion microscopy.Anal. Chem. 61, 2690–5.

    PubMed  Google Scholar 

  • Barth, R. F., Soloway, A. H. &Fairchild, R. G. (1990) Boron neutron capture therapy of cancers.Cancer Res. 50, 1061–70.

    PubMed  Google Scholar 

  • Bendayan, M., Barth, R. F., Gingras, D., Londono, I., Robinson, P. T., Alam, F., Adams, D. M. &Mattiazzi, L. (1989) Electron spectroscopic imaging for high-resolution immunocytochemistry: use of boronated protein A.J. Histochem. Cytochem. 37, 573–80.

    PubMed  Google Scholar 

  • Carpenter, B. S. &Lafleur, P. D. (1974) Nitrogen determinations in biological material by the nuclear track technique.Anal. Chem. 46, 1112–3.

    PubMed  Google Scholar 

  • Clarke, W. B., Koekebakker, M., Barr, R. D., Downing, R. G. &Fleming, R. F. (1987) Analysis of ultratrace lithium and boron by neutron activation and mass-spectrometric measurement of3He and4He.Int. J. Radiat. Appl. Instrum. 38, 735–43.

    Google Scholar 

  • Edwards, L. C. (1956) Autoradiography by neutron activation: the cellular distribution of boron 10 in the transplanted mouse brain tumor.Internat J. Appl. Radiat. Isot. 1, 184–90.

    Google Scholar 

  • Ficq, A. (1951) Autoradiographie par neutrons: dosage du lithium dans les embryons d'amphibiens.C. R. Acad. Sci. Paris 233, 1684–5.

    PubMed  Google Scholar 

  • Gabel, D., Holstein, H., Larsson, B., Gille, L., Ericson, G., Sacker, D., Som, P. &Fairchild, R. G. (1987) Quantitative neutron capture radiography for studying the biodistribution of tumor-seeking boron-containing compounds.Cancer Res. 47, 5451–4.

    PubMed  Google Scholar 

  • Garin, A. &Thellier, M. (1958) Methode de microdétermination du bore dans une solution ou un tissu végétal par activation aux neutrons lents.Bull. Microscopie Appl. 8, 129–48.

    Google Scholar 

  • Gelinas, Y., Ferraris, J. & Schmit, J. P. (1989) Inorganic fingerprint of rat tissues.Proc. 37th ASMS Conf. Mass Spectr. Allied Top. Miami Beach, FL. pp. 178–9.

  • Hennequin, E., Laurent-Pettersson, M., Fernandez, T., Larsson, B. &Thellier, M. (1990) Nitrogen imaging in histological sections, using neutron-capture radiography.J. Trace Microprobe Tech. 7, 221–46.

    Google Scholar 

  • Hunt, C. D. (1989) Dietary boron modified the effects of magnesium and molybdenum on mineral metabolism in the cholecalciferol-deficient chick.Biol. Trace Element. Res. 22, 201–20.

    Google Scholar 

  • Iyengar, G. V. (1989)Elemental Analysis of Biological Systems. Florida: CRC Press Inc.

    Google Scholar 

  • Jimenez, R., Loria, L. G., Gallardo, M. &Thellier, M. (1988). Detection and imaging of boron in plant tissues, for agronomical purposes in Costa Rica, using small Cf 252 neutron sources.C. R. Acad. Sci. Paris série III,306, 583–90.

    Google Scholar 

  • Lamotte, M. (1967)Initiation aux Méthodes Statistiques en Biologie. Paris: Masson.

    Google Scholar 

  • Laurent Pettersson, M., Courel, M.-N., Girard, N., Abraham, R., Gabel, D., Thellier, M. &Delpech, B. (1990) Immunoreactivity of boronated antibodies.J. Immunol Meth. 126, 95–102.

    Google Scholar 

  • Magour, S., Schramel, P., Ovcar, J. &Mäser, H. (1982) Uptake and distribution of boron in rats: interaction with ethanol and hexabarbital in the brain.Arh. Environm. Contam. Toxicol. 11, 521–5.

    Google Scholar 

  • Massie, H. R., Aiello, V. R., Shumway, M. E. &Armstrong, T. (1990) Calcium, iron, copper, boron, collagen, and density changes in bone with aging in C57BL/6J male mice.Exp. Gerontol. 25, 469–81.

    PubMed  Google Scholar 

  • Morel, F. &Imbert-Teboul, M. (1980) Mécanismes de concentration de l'urine par le rein. InLa Function Rénale Acquisitions et Perspectives (edited byBonvalet, J.-P.) pp. 125–39. Paris: Flammarion.

    Google Scholar 

  • Nielsen, F. H. (1990) Studies on the relationship between boron and magnesium which possibly affects the formation and maintenance of bones.Magnesium Trace Elem. 9, 61–9.

    Google Scholar 

  • Nielsen, F. H. (1991) Nutritional requirements for boron, silicon, vanadium, nickel, and arsenic: current knowledge and speculation.FASEB J. 5, 2661–7.

    PubMed  Google Scholar 

  • Pettersson, O.-A. Svensson, P., Larsson, B. & Grusell, E. Studsvik thermal neutron facility. InNeutron Capture Therapy for Cancer (edited byAllen, B. J.). Plenum (in press).

  • Shorrocks, V. M. (1991) Behaviour, function and significance of boron in agriculture. London; Borax Consolidated Ltd.

    Google Scholar 

  • Shuler, T. R., Pootrakul, P., Yarnsukon, P. &Nielsen, F. H. (1990) Effect of thalassemia/hemoglobin E disease on macro, trace, and ultratrace element concentrations in human tissue.J. Trace Elem. Exp. Med. 3, 31–43.

    Google Scholar 

  • Thellier, M., Duval, Y. &Demarty, M. (1979) Borate exchanges ofLemna minor L. as studied with the help of the enriched stable isotopes and a (n, α) nuclear reaction.Plant Physiol. 63, 283–6.

    Google Scholar 

  • Thellier, M., Heurteaux, C. &Wissocq, J.-C. (1980a) Quantitative study of the distribution of lithium in the mouse brain for various doses of lithium given to the animal.Brain Res. 199, 175–96.

    PubMed  Google Scholar 

  • Thellier, M., Wissocq, J.-C. &Heurteaux, C. (1980b) Quantitative microlocalisation of lithium in the brain by a (n, α) nuclear reaction.Nature 283, 299–302.

    PubMed  Google Scholar 

  • Thellier, M., Hennequin, E., Heurteaux, C., Martini, F., Pettersson, M., Fernandez, T. &Wisocq, J.-C. (1988) Quantitative estimations in neutron capture radiography.Nucl. Instr. Meth. Phys. Res. B30, 567–79.

    Google Scholar 

  • Walker, F. W., Miller, D. G. &Feiner, F. (1983)Chart of the Nuclides, 13th edn. San Jose, Florida: General Electrical Company.

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire d'Oncologie Moléculaire, Centre Henri-Becquerel, rue d'Amiens, F-76038, Rouen Cedex, France

    Maria Laurent-Pettersson & Bertrand Delpech

  2. Laboratoire Echanges Cellulaires, URA 203 CNRS, Faculté des Sciences et Techniques, BP 118, F-76134, Mont-Saint-Aignan Cedex, France

    Maria Laurent-Pettersson & Michel Thellier

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  1. Maria Laurent-Pettersson
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  2. Bertrand Delpech
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  3. Michel Thellier
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Laurent-Pettersson, M., Delpech, B. & Thellier, M. The mapping of natural boron in histological sections of mouse tissues by the use of neutron-capture radiography. Histochem J 24, 939–950 (1992). https://doi.org/10.1007/BF01046499

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